@inproceedings{9d1bea8330604de393b967f1d3208f6d,
title = "Zanamivir immobilized magnetic beads for voltammetric measurement of neuraminidase at gold-modified boron doped diamond electrode",
abstract = "Biomolecule modified magnetic beads has been widely used in separation and sensing process. This study used streptavidin modified magnetic beads to immobilize biotin modified zanamivir. Biotin-streptavidin affinity facilitates immobilization of zanamivir on magnetic beads. Then interaction of zanamivir and neuraminidase was adopted as basic for enzyme detection. Detection of neuraminidase was performed at gold modified BDD using cyclic voltammetry technique. The measurement was carried out based on alteration of electrochemical signals of working electrode as neuraminidase response. The result showed that zanamivir was successfully immobilized on magnetic beads. The optimum amount of magnetic beads for zanamivir immobilization was 120 ug. Linear responses of neuraminidase were detected in concentration range of 0-15 mU. Detection limit (LOD) of measurement was 2.32 mU (R2 = 0.959) with precision as % RSD of 1.41%. Measurement of neuraminidase on magnetic beads could be also performed in the presence of mucin matrix. The linearity range was 0-8 mU with LOD of 0.64 mU (R2 = 0.950) and % RSD of 7.25%.",
author = "Wahyuni, {Wulan Tri} and Anggraningrum, {Ivandini Tribidasari} and Endang Saefudin and Yasuaki Einaga",
note = "Publisher Copyright: {\textcopyright} 2016 Author(s).; 1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 ; Conference date: 03-11-2015 Through 04-11-2015",
year = "2016",
month = apr,
day = "19",
doi = "10.1063/1.4946958",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Terry Mart and Djoko Triyono",
booktitle = "International Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015",
}