Zanamivir immobilized magnetic beads for voltammetric measurement of neuraminidase at gold-modified boron doped diamond electrode

Wulan Tri Wahyuni, Ivandini Tribidasari Anggraningrum, Endang Saefudin, Yasuaki Einaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomolecule modified magnetic beads has been widely used in separation and sensing process. This study used streptavidin modified magnetic beads to immobilize biotin modified zanamivir. Biotin-streptavidin affinity facilitates immobilization of zanamivir on magnetic beads. Then interaction of zanamivir and neuraminidase was adopted as basic for enzyme detection. Detection of neuraminidase was performed at gold modified BDD using cyclic voltammetry technique. The measurement was carried out based on alteration of electrochemical signals of working electrode as neuraminidase response. The result showed that zanamivir was successfully immobilized on magnetic beads. The optimum amount of magnetic beads for zanamivir immobilization was 120 ug. Linear responses of neuraminidase were detected in concentration range of 0-15 mU. Detection limit (LOD) of measurement was 2.32 mU (R2 = 0.959) with precision as % RSD of 1.41%. Measurement of neuraminidase on magnetic beads could be also performed in the presence of mucin matrix. The linearity range was 0-8 mU with LOD of 0.64 mU (R2 = 0.950) and % RSD of 7.25%.

Original languageEnglish
Title of host publicationInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
Subtitle of host publicationProceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
EditorsTerry Mart, Djoko Triyono
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413764
DOIs
Publication statusPublished - 19 Apr 2016
Event1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
Duration: 3 Nov 20154 Nov 2015

Publication series

NameAIP Conference Proceedings
Volume1729
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
CountryIndonesia
CityDepok
Period3/11/154/11/15

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    Wahyuni, W. T., Anggraningrum, I. T., Saefudin, E., & Einaga, Y. (2016). Zanamivir immobilized magnetic beads for voltammetric measurement of neuraminidase at gold-modified boron doped diamond electrode. In T. Mart, & D. Triyono (Eds.), International Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015: Proceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences [020055] (AIP Conference Proceedings; Vol. 1729). American Institute of Physics Inc.. https://doi.org/10.1063/1.4946958