Whole-genome sequencing analysis of multidrug-resistant Mycobacterium tuberculosis from Java, Indonesia

Tryna Tania, Pratiwi Sudarmono, R. Lia Kusumawati, Andriansjah Rukmana, Wahyu Agung Pratama, Sanjib Mani Regmi, Orawee Kaewprasert, Angkana Chaiprasert, Virasakdi Chongsuvivatwong, Kiatichai Faksri

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction. Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally, including in Indonesia. Whole-genome sequencing (WGS) analysis has rarely been used for the study of TB and MDR-TB in Indonesia.Aim. We evaluated the use of WGS for drug-susceptibility testing (DST) and to investigate the population structure of drug-resistant Mycobacterium tuberculosis in Java, Indonesia.Methodology. Thirty suspected MDR-TB isolates were subjected to MGIT 960 system (MGIT)-based DST and to WGS. Phylogenetic analysis was done using the WGS data. Results obtained using MGIT-based DST and WGS-based DST were compared.Results. Agreement between WGS and MGIT was 93.33 % for rifampicin, 83.33 % for isoniazid and 76.67 % for streptomycin but only 63.33 % for ethambutol. Moderate WGS-MGIT agreement was found for second-line drugs including amikacin, kanamycin and fluoroquinolone (73.33-76.67 %). MDR-TB was more common in isolates of the East Asian Lineage (63.3%). No evidence of clonal transmission of DR-TB was found among members of the tested population.Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia.

Original languageEnglish
Pages (from-to)1013-1019
Number of pages7
JournalJournal of Medical Microbiology
Volume69
Issue number7
DOIs
Publication statusPublished - 1 Jul 2020

Keywords

  • Indonesia
  • MDR-TB
  • Mycobacterium tuberculosis
  • whole-genome sequencing

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