TY - JOUR
T1 - Whole-genome sequencing analysis of multidrug-resistant Mycobacterium tuberculosis from Java, Indonesia
AU - Tania, Tryna
AU - Sudarmono, Pratiwi
AU - Kusumawati, R. Lia
AU - Rukmana, Andriansjah
AU - Pratama, Wahyu Agung
AU - Regmi, Sanjib Mani
AU - Kaewprasert, Orawee
AU - Chaiprasert, Angkana
AU - Chongsuvivatwong, Virasakdi
AU - Faksri, Kiatichai
N1 - Publisher Copyright:
001221 © 2020 The Authors.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Introduction. Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally, including in Indonesia. Whole-genome sequencing (WGS) analysis has rarely been used for the study of TB and MDR-TB in Indonesia.Aim. We evaluated the use of WGS for drug-susceptibility testing (DST) and to investigate the population structure of drug-resistant Mycobacterium tuberculosis in Java, Indonesia.Methodology. Thirty suspected MDR-TB isolates were subjected to MGIT 960 system (MGIT)-based DST and to WGS. Phylogenetic analysis was done using the WGS data. Results obtained using MGIT-based DST and WGS-based DST were compared.Results. Agreement between WGS and MGIT was 93.33 % for rifampicin, 83.33 % for isoniazid and 76.67 % for streptomycin but only 63.33 % for ethambutol. Moderate WGS-MGIT agreement was found for second-line drugs including amikacin, kanamycin and fluoroquinolone (73.33-76.67 %). MDR-TB was more common in isolates of the East Asian Lineage (63.3%). No evidence of clonal transmission of DR-TB was found among members of the tested population.Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia.
AB - Introduction. Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally, including in Indonesia. Whole-genome sequencing (WGS) analysis has rarely been used for the study of TB and MDR-TB in Indonesia.Aim. We evaluated the use of WGS for drug-susceptibility testing (DST) and to investigate the population structure of drug-resistant Mycobacterium tuberculosis in Java, Indonesia.Methodology. Thirty suspected MDR-TB isolates were subjected to MGIT 960 system (MGIT)-based DST and to WGS. Phylogenetic analysis was done using the WGS data. Results obtained using MGIT-based DST and WGS-based DST were compared.Results. Agreement between WGS and MGIT was 93.33 % for rifampicin, 83.33 % for isoniazid and 76.67 % for streptomycin but only 63.33 % for ethambutol. Moderate WGS-MGIT agreement was found for second-line drugs including amikacin, kanamycin and fluoroquinolone (73.33-76.67 %). MDR-TB was more common in isolates of the East Asian Lineage (63.3%). No evidence of clonal transmission of DR-TB was found among members of the tested population.Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia.
KW - Indonesia
KW - MDR-TB
KW - Mycobacterium tuberculosis
KW - whole-genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85088880861&partnerID=8YFLogxK
U2 - 10.1099/jmm.0.001221
DO - 10.1099/jmm.0.001221
M3 - Article
C2 - 32579102
AN - SCOPUS:85088880861
SN - 0022-2615
VL - 69
SP - 1013
EP - 1019
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 7
ER -