TY - JOUR
T1 - Wharton's Jelly Mesenchymal Stem Cells
T2 - Differentiation Capacity Showing its Role in Bone Tissue Engineering
AU - Rizal, Rizal
AU - Syaidah, Rahimi
AU - Evelyn, Evelyn
AU - Hafizh, Alif Muhammad
AU - Frederich, Josh
N1 - Funding Information:
This study was supported by a grant from Universitas Indonesia, PUTI Prosiding 2020, contract no. NKB-912/UN.RST/HKP.05.00/2020.
Publisher Copyright:
© 2020. All Rights Reserved.
PY - 2020/11/20
Y1 - 2020/11/20
N2 - Wharton's jelly mesenchymal stem cells (WJ-MSCs) is one of the best sources of mesenchymal stem cells (MSCs) that suggest both embryonic and adult stem cell characteristics. Before being applied in clinical application, the isolated MSCs should be tested to assess their quality, including differentiation capacity, phenotype characterization, and morphological appearance. This research aims to quantify the differentiation capacity of WJ-MSCs isolated using explant method. The WJ-MSCs cells were grown out from Wharton's jelly tissue and the isolated cells adhered in T25 plastic flask. The isolated cells expressed high amount of MSC surface marker which are CD105 (99.97±0.06%), CD73 (99.97±0.06%), and CD90 (99.12±0.25%). The cells can be differentiated into adipocytes, chondrocytes, and osteocytes. The quantification showed that the amount of mineralization in osteoblastogenesis, production of lipid droplet in adipogenic differentiation, and production of glycosaminoglycan in chondrogenesis were noticeably higher in differentiated cells than non-differentiated cells. In conclusion, the isolated cells fulfill the minimum criteria of MSCs that can be used in research or clinical application. The great differentiation capacity of the cells into osteocytes and chondrocytes indicate that the cells are suitable in bone tissue engineering application, both for research and clinical application.
AB - Wharton's jelly mesenchymal stem cells (WJ-MSCs) is one of the best sources of mesenchymal stem cells (MSCs) that suggest both embryonic and adult stem cell characteristics. Before being applied in clinical application, the isolated MSCs should be tested to assess their quality, including differentiation capacity, phenotype characterization, and morphological appearance. This research aims to quantify the differentiation capacity of WJ-MSCs isolated using explant method. The WJ-MSCs cells were grown out from Wharton's jelly tissue and the isolated cells adhered in T25 plastic flask. The isolated cells expressed high amount of MSC surface marker which are CD105 (99.97±0.06%), CD73 (99.97±0.06%), and CD90 (99.12±0.25%). The cells can be differentiated into adipocytes, chondrocytes, and osteocytes. The quantification showed that the amount of mineralization in osteoblastogenesis, production of lipid droplet in adipogenic differentiation, and production of glycosaminoglycan in chondrogenesis were noticeably higher in differentiated cells than non-differentiated cells. In conclusion, the isolated cells fulfill the minimum criteria of MSCs that can be used in research or clinical application. The great differentiation capacity of the cells into osteocytes and chondrocytes indicate that the cells are suitable in bone tissue engineering application, both for research and clinical application.
KW - Adipocytes
KW - Chondrocytes
KW - Differentiation capacity
KW - Osteocytes
KW - WJ-MSCs
UR - http://www.scopus.com/inward/record.url?scp=85097676613&partnerID=8YFLogxK
U2 - 10.14716/ijtech.v11i5.4309
DO - 10.14716/ijtech.v11i5.4309
M3 - Article
AN - SCOPUS:85097676613
SN - 2087-2100
VL - 11
SP - 1005
EP - 1014
JO - International Journal of Technology
JF - International Journal of Technology
IS - 5
ER -