TY - JOUR
T1 - Visualization of Microlejeunea ulicina by using Transmission Electron Microscopy
AU - Nabella, D. M.
AU - Yenti, D. A.R.
AU - Putrika, A.
AU - Dwiranti, A.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2019/10/9
Y1 - 2019/10/9
N2 - Oil bodies are highly distinctive organelle uniquely found in liverworts. Besides the importance of this structure in taxonomy, many of the secondary metabolites contained within the oil bodies are also of the great value as potential sources of medicines. Thus, the investigation of liverwort ultrastructure is essential to give an insight into the oil bodies structure and function. Microlejeunea ulicina is one species of the leafy liverworts found in Universitas Indonesia that has not previously been reported to exist in Java island. The aim of this study is to investigate the ultrastructure of M. ulicina by using Transmission Electron Microscopy (TEM). M.ulicina were fixed with 2.5% glutaraldehyde, post-fixed with 4% Osmium tetroxide, dehydrated with Ethanol series, and then subjected to resin embedding. After samples embedding, sectioning by ultramicrotome was performed. Post-staining with Uranyl acetate and Lead Citrate was carried out following sectioning and the samples were finally observed by TEM. Our results showed the ultrastructure of M. ulicina for the first time. The structure of oil bodies could be distinguished with the other parts of the cells. Further analysis of the oil bodies by comparing our data with other species of leafy liverwort in Universitas Indonesia will be beneficial for the species identification as well as the exploration of their secondary metabolites.
AB - Oil bodies are highly distinctive organelle uniquely found in liverworts. Besides the importance of this structure in taxonomy, many of the secondary metabolites contained within the oil bodies are also of the great value as potential sources of medicines. Thus, the investigation of liverwort ultrastructure is essential to give an insight into the oil bodies structure and function. Microlejeunea ulicina is one species of the leafy liverworts found in Universitas Indonesia that has not previously been reported to exist in Java island. The aim of this study is to investigate the ultrastructure of M. ulicina by using Transmission Electron Microscopy (TEM). M.ulicina were fixed with 2.5% glutaraldehyde, post-fixed with 4% Osmium tetroxide, dehydrated with Ethanol series, and then subjected to resin embedding. After samples embedding, sectioning by ultramicrotome was performed. Post-staining with Uranyl acetate and Lead Citrate was carried out following sectioning and the samples were finally observed by TEM. Our results showed the ultrastructure of M. ulicina for the first time. The structure of oil bodies could be distinguished with the other parts of the cells. Further analysis of the oil bodies by comparing our data with other species of leafy liverwort in Universitas Indonesia will be beneficial for the species identification as well as the exploration of their secondary metabolites.
UR - http://www.scopus.com/inward/record.url?scp=85074787324&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1245/1/012004
DO - 10.1088/1742-6596/1245/1/012004
M3 - Conference article
AN - SCOPUS:85074787324
SN - 1742-6588
VL - 1245
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012004
T2 - 7th Biannual International Conference on Mathematics and Natural Sciences, ICMNS 2018
Y2 - 2 November 2018 through 3 November 2018
ER -