Viability of Metarhizium majus UICC 295 after preservation by freezing at -80°C

D. C.A.F. Sari, A. Oetari, W. Sjamsuridzal

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Reliable preservation method is required to ensure the integrity of entomopathogenic features of fungus entomopathogen. This study evaluated the viability of entomopathogenic fungus Metarhizium majus UICC 295 maintained in freezing preservation at -80°C by using two different cryoprotectants. Metarhizium majus UICC 295 was cultivated in Saboraud Dextrose with Yeast Extract Agar (SDAY) and SDAY without 10% (w/v) cricket powder. Two different cryoprotectants were used, 10% (v/v) glycerol and 10% (v/v) glycerol with 5% (w/v) trehalose. Sterile water was used as control. The initial cell number was (5.04x106) CFU/mL in SDAY, and (2.5x107) CFU/mL in SDAY with cricket powder. The spore and hyphal suspensions were pre-incubated at 4°C for one hour to allow the cryoprotectants to diffuse into the cell, before storing at -80°C. Viability was evaluated by enumeration using total plate count (TPC) on SDAY and quantifying the percentage of viable cell number at 1, 14, and 30 days of preservation. Each treatment was replicated three times. The results showed that viability of spores and hyphae of M. majus was maintained in this study. The fungal strain was able to withstand the extreme temperature. Adequate recovery of spores and hyphae could be obtained without using a cryoprotectant. Highest percentage of viability (62.10%) with the cell number (3.13x106CFU/mL) was obtained from the sample prepared from SDAY, which was maintained in sterile water. The present study showed that the preservation method at -80°C in sterile water allowed the recovery of the fungus, while preservation at - 80°C in cryoprotectants was not effective and resulted in low recovery of the fungus.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

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