Veillonella nakazawae sp. Nov., an anaerobic gram-negative coccus isolated from the oral cavity of japanese children

Izumi Mashima, Citra F. Theodorea, Ariadna A. Djais, Tadao Kunihiro, Yoshiaki Kawamura, Maiko Otomo, Masato Saitoh, Riyoko Tamai, Yusuke Kiyoura

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1 Citation (Scopus)

Abstract

Two strains of previously unknown Gram-negative cocci, T1-7T and S6-16, were isolated from the oral cavity of healthy Japanese children. The two strains showed atypical phenotypic characteristics of members of the genus Veillonella, including catalase production. Sequencing of their 16S rRNA genes confirmed that they belong to genus Veillonella. Under anaerobic con-ditions, the two strains produced acetic acid and propionic acid as metabolic end-products in a trypticase–yeast extract–haemin medium containing 1% (w/v) glucose, 1% (w/v) fructose and 1% (v/v) sodium lactate. Comparative analysis of the 16S rRNA, dnaK, rpoB and gltA gene sequences revealed that the two strains are phylogenetically homogeneous and comprise a distinct, novel lineage within the genus Veillonella. The sequences from the two strains shared the highest similarity, at 99.9, 95.8, 96.9 and 96.7%, using the partial 16S rRNA, dnaK, rpoB and gltA gene sequences, respectively, with the type strains of the two most closely related species, Veillonella dispar ATCC 17748T and Veillonella infantium JCM 31738T. Furthermore, strain T1-7T shared the highest average nucleotide identity (ANI) value (94.06%) with type strain of the most closely related species, V. infantium. At the same time, strain T1-7T showed the highest digital DNA–DNA hybridization (dDDH) value (55.5%) with the type strain of V. infantium. The two strains reported in this study were distinguished from the previously reported species from the genus Veillonella based on catalase production, partial dnaK, rpoB and gltA sequences, average ANI and dDDH values. Based on these observations, the two strains represent a novel species, for which the name Veillonella nakazawae sp. nov. is proposed. The type strain is T1-7T (JCM 33966T=CCUG 74597T).

Original languageEnglish
Article number004583
Pages (from-to)1-7
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume71
Issue number1
DOIs
Publication statusPublished - 2021

Keywords

  • Japanese children
  • Oral anaerobic Gram-negative coccus
  • Saliva
  • Tongue biofilm
  • Veillonella nakazawae sp. nov.

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