TY - JOUR
T1 - Veillonella infantium sp. Nov., an anaerobic, gram-stain-negative coccus isolated from tongue biofilm of a thai child
AU - Mashima, Izumi
AU - Liao, Yu Chieh
AU - Miyakawa, Hiroshi
AU - Theodorea, Citra F.
AU - Thawboon, Boonyanit
AU - Thaweboon, Sroisiri
AU - Scannapieco, Frank A.
AU - Nakazawa, Futoshi
N1 - Funding Information:
This study was supported in part by grants-in-aid from the Japan Society for the Promotion of Science (JSPS) Fellows (15J30007), Scientific Research from KAKENHI (Grant number: 26462793), the 2014– 2015 and 2016–2017 Research Project of the Research Institute of Personalized Health Sciences, Health Sciences University of Hokkaido, research grant 2016 and 2017 from the Institute for Fermentation, Osaka, and 2017 Mahidol University Faculty of Dentistry Grant (Grant number: 0517.0321/00100).
Funding Information:
This study was supported in part by grants-in-aid from the Japan Society for the Promotion of Science JSPS Fellows (15J30007), Scientific Research from KAKENHI (Grant number: 26462793), the 2014-2015 and 2016–2017 Research Project of the Research Institute of Personalized Health Sciences, Health Sciences University of Hokkaido, research grant 2016 and 2017 from the Institute for Fermentation, Osaka, and 2017 Mahidol University Faculty of Dentistry Grant (Grant number: 0517.0321/00100).
Publisher Copyright:
© 2018 IUMS.
PY - 2018/4
Y1 - 2018/4
N2 - A strain of a novel anaerobic, Gram-stain-negative coccus was isolated from the tongue biofilm of a Thai child. This strain was shown, at the phenotypic level and based on 16S rRNA gene sequencing, to be a member of the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus Veillonella. The novel strain showed 99.8, 95.1 and 95.9% similarity to partial 16S rRNA, dnaK and rpoB gene sequences, respectively, to the type strains of the two most closely related species, Veillonella dispar ATCC 17748Tand Veillonella tobetsuensis ATCC BAA-2400T. The novel strain could be discriminated from previously reported species of the genus Veillonella based on partial dnaK and rpoB gene sequencing and average nucleotide identity values. The major acid end-product produced by this strain was acetic acid under anaerobic conditions in trypticase-yeast extract-haemin with 1% (w/v) glucose or fructose medium. Lactate was fermented to acetic acid and propionic acid. Based on these observations, this strain represents a novel species, for which the name Veillonella infantium sp. nov. is proposed. The type strain is T11011-4T (=JCM 31738T=TSD-88T).
AB - A strain of a novel anaerobic, Gram-stain-negative coccus was isolated from the tongue biofilm of a Thai child. This strain was shown, at the phenotypic level and based on 16S rRNA gene sequencing, to be a member of the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus Veillonella. The novel strain showed 99.8, 95.1 and 95.9% similarity to partial 16S rRNA, dnaK and rpoB gene sequences, respectively, to the type strains of the two most closely related species, Veillonella dispar ATCC 17748Tand Veillonella tobetsuensis ATCC BAA-2400T. The novel strain could be discriminated from previously reported species of the genus Veillonella based on partial dnaK and rpoB gene sequencing and average nucleotide identity values. The major acid end-product produced by this strain was acetic acid under anaerobic conditions in trypticase-yeast extract-haemin with 1% (w/v) glucose or fructose medium. Lactate was fermented to acetic acid and propionic acid. Based on these observations, this strain represents a novel species, for which the name Veillonella infantium sp. nov. is proposed. The type strain is T11011-4T (=JCM 31738T=TSD-88T).
KW - A Thai child
KW - Oral anaerobic Gram-stain-negative cocci
KW - Tongue biofilm
KW - Veillonella infantium sp. nov
UR - http://www.scopus.com/inward/record.url?scp=85044963442&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002632
DO - 10.1099/ijsem.0.002632
M3 - Article
C2 - 29458564
AN - SCOPUS:85044963442
SN - 1466-5026
VL - 68
SP - 1101
EP - 1106
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 4
M1 - 002632
ER -