TY - JOUR
T1 - Validation of Rosmarinic Acid Quantification using High-Performance Liquid Chromatography in Various Plants
AU - Syarifah, Andiri Niza
AU - Suryadi, Herman
AU - Mun’im, Abdul
N1 - Funding Information:
The authors would like to acknowledge a research funding from the Republic of Indonesia's Ministry of Research, Technology, and Higher Education, contract number NKB-374/UN2.RST/HKP.05.00/2021.
Publisher Copyright:
© 2022 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2022/1
Y1 - 2022/1
N2 - Introduction: Rosmarinic acid has been utilized in traditional medicine as antioxidant, antiinflammation, anticancer and antibacterial. In order to control the herbal quality, validation of rosmarinic acid determination using high-performance liquid chromatography was developed. The objective of this report was to validate an HPLC technique for assessing rosmarinic acid levels. and application that method to determine rosmarinic acid in Rosmarinus officinalis, Symphytum officinale, Mentha piperita, Orthosiphon stamineus and Salvia officinale. Methods: The chromatographic separation was carried out on a reversed-phase C18 column with a mobile phase of 0,1% formic acid and acetonitrile and an isocratic elution at a flow rate of 0,5 mL/min. The wavelength for detection was set to 330 nm. The method has been validated for precision, accuracy, linearity, limit of detection, and limit of quantitation. Result: The concentration response of the detector was linear, with a coefficient of determination of 0.9933. The HPLC technique had an accuracy of 101,00 ± 6,43%. The precision was 6,36% when expressed as a coefficient of variation (CV). The highest level of rosmarinic acid was 214,86 ± 0,60 µg/mL in Rosmarinus officinalis extract. Conclusion: The HPLC method was valid to analyse rosmarinic acid level. The method can be applied in routine determination of rosmarinic acid of phytopharmaceutical products.
AB - Introduction: Rosmarinic acid has been utilized in traditional medicine as antioxidant, antiinflammation, anticancer and antibacterial. In order to control the herbal quality, validation of rosmarinic acid determination using high-performance liquid chromatography was developed. The objective of this report was to validate an HPLC technique for assessing rosmarinic acid levels. and application that method to determine rosmarinic acid in Rosmarinus officinalis, Symphytum officinale, Mentha piperita, Orthosiphon stamineus and Salvia officinale. Methods: The chromatographic separation was carried out on a reversed-phase C18 column with a mobile phase of 0,1% formic acid and acetonitrile and an isocratic elution at a flow rate of 0,5 mL/min. The wavelength for detection was set to 330 nm. The method has been validated for precision, accuracy, linearity, limit of detection, and limit of quantitation. Result: The concentration response of the detector was linear, with a coefficient of determination of 0.9933. The HPLC technique had an accuracy of 101,00 ± 6,43%. The precision was 6,36% when expressed as a coefficient of variation (CV). The highest level of rosmarinic acid was 214,86 ± 0,60 µg/mL in Rosmarinus officinalis extract. Conclusion: The HPLC method was valid to analyse rosmarinic acid level. The method can be applied in routine determination of rosmarinic acid of phytopharmaceutical products.
KW - HPLC
KW - Laminaceae Borraginaceae
KW - Rosmarinic acid
UR - http://www.scopus.com/inward/record.url?scp=85125735391&partnerID=8YFLogxK
U2 - 10.5530/pj.2022.14.22
DO - 10.5530/pj.2022.14.22
M3 - Article
AN - SCOPUS:85125735391
SN - 0975-3575
VL - 14
SP - 165
EP - 171
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 1
ER -