Utilization of sesame seed sprout biomass as lipase source to hydrolyse palm oil

Lipase is known enzyme for its ability to hydrolyse triglycerides into free fatty acid. There are so much sources od lipase such as plants, microbes, and animal. But, lipase from plant seems interesting owing to its huge amount than microbes' lipase and less harm than animal's lipase. Many experts have proved that vegetable lipase is a potential biocatalyst so that lipase from sesame seed and sesame sprout was investigated in this research. Lipase which was obtained from each sources were then poured with 0.3 g PVA and 40% palm oil in demineral water. The lipase-palm oil solutions were then titrated with 0.05 M NaOH obtaining hydrolysis percentage. Based on the research, sesame seed supernatant produces 1.36 mmol FFA with 39% hydrolysis. Sesame sprout extract produces 1.37 mmol FFA with 39.14% hydrolysis. Those samples also contain lauric acid, miristic acid, palmitic acid, oleic acid and stearic acid which detected by GC instrument.