Utilization of Nicotiana tobacum 's extract for mosquito extermination with fogging method

The number of Dengue Hemorrhagic Fever (DHF) victims in DKI Jakarta province continues to increase, despite many efforts have been made to control the spread of DHF. An example of permissive control to tackle DHF issue is the use of chemical-based fogging for fumigation. However, insecticide has an active compound that causes mosquito resistance and it has been discovered to have side effects on the human body. Its application in fogging also requires petroleum fuels as smoke-making agent. This study intends to substitute fluids formulation in fogging formula. This formulation aims to create liquid fogging products that are free from pyrethroid and petroleum, so fumigation becomes safer for its users. The research was done by formulating tobacco leaf extract as an active insecticide substance with a solvent from propylene glycol and glycerin as a smoke-making agent. The independent variables in this study were the concentration of the active substance and the ratio of solvent (PG:VG). Tests that will be conducted on the samples are LC₅₀ toxicity test, physical stability test, organoleptic test, GCMS test. The results of the toxicity test with pyrolysis extract showed that with a concentration of 10%-20% has not reached LC>₅₀, the concentration of 30% reached LC₅₀ in the 9^th minute. The concentration of 40% reached LC₅₀ in the 6^th minute and the concentration of 50% reached LC₅₀ in the 5^th minute. For tobacco extract with extraction method, the concentration of 5%-20% has not reached LC₅₀. The concentration of 30% reaches LC₅₀ in the 9^th minute. The concentration of 40% reached LC₅₀ in the 4^th minute and the concentration of 50% in the 3^rd minute.