Utilization of lionfish (Pterois volitans) venomous spines with effective purification as an alternative antiretroviral HIV/AIDS

Andy Noorsaman Sommeng, Muhammad Akbar Buana Tafsili, Mikael Januardi Ginting, Muhamad Sahlan, Heri Hermansyah, Anondho Wijanarko

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Lionfish (Pterois volitans) are a fish that live in the Indo-Pacific region. Today, Lionfish have an increasing population and invaded up to the Atlantic Ocean. The invasion of Lionfish was not accompanied by the presence of predators, thus causing damage to the ecosystem and food chain. One of the control efforts that possible be done is the utilization of venomous spines of Lionfish that contains Phospholipase A2 known to have antiretroviral activity. So it has the potential to be used as an alternative treatment of HIV/AIDS infection. This research aims to increase the purity and specific activity of Phospholipase A2 from venomous spines of Lionfish in the Java Sea, Indonesia. Phospholipase A2 isolated from Lionfish venom from the waters of the Java Sea with Salting-out method using Ammonium Sulfate on 20% saturation level and the addition of 0.5 mL of Caprylic Acid by heating 60 °C for 20 minutes has the highest enzymes activity and purity. The specific activities reaching 6.48 units/μg, safe tested on human cells with 28.36 ppm inhibition measured with LC50 and also can inhibit infected cell growth with 98.13% inhibition degree.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/TerritoryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • Antiretroviral
  • Caprylic acid
  • Lionfish
  • Phospholipase A2
  • Venomous Spines

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