Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity

Arif Arrahman, Efendy Sigalingging, Elsa Trinovita, Fadlina Chany Saputri, A. Mun’Im

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/ solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor.

Original languageEnglish
Article numbere18115
Pages (from-to)1-11
Number of pages11
JournalBrazilian Journal of Pharmaceutical Sciences
Volume56
DOIs
Publication statusPublished - 2020

Keywords

  • IL-MAE
  • Tyrosinase
  • [Bmim]BF Ionic liquid
  • γ-oryzanol

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