Currently, the main energy source is heavily dependent on fossil energy. The current transportation technology also uses fossil-derived energy sources to make vehicle engines are ignited. In addition, the electricity that is currently enjoyed by billions of people resulted enormously from the use of fossil energy. Limitations of existing fossil energy sources and the issue of global warming have led many to expand on renewable energy and energy conservation to maintain energy availability. One of alternative energy source that is currently being developed is the use of bioethanol as a mixture or replacement of fossil fuel. This paper is a series of advanced research on the utilization of low-grade bioethanol from SI carburetor engine to the SI Injection engine. The research phases are begun by designing compact distillator on a dynamic engine, and then optimization is done on the compact distillator and followed by performance testing and exhaust gas emission. The low-grade bioethanol distillation process is carried out independently by utilizing heat from flue gas to produce high-grade bioethanol. The research was then developed on an SI injection engine to determine the performance and exhaust emissions in some bioethanol fuel mixtures with gasoline. From this continuation study was obtained some results, the first is the use of appropriate fuel mixing method in each study. In addition, optimization performed on the compact distillator will result in an increase in distillation rate and concentration of ethanol of 96.91%. And the last is an analysis of the performance and exhaust emissions generated in each study.