Use of microcrystalline cellulose and carboxymethyl cellulose for the detection of cellulolytic fungi from old Chinese manuscripts

Ariyanti Oetari, R. Fitri, M. K. Rachmania, Wellyzar Sjamsuridzal

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Historical materials made of paper are especially susceptible to fungal deterioration. Most of the fungi that associated with paper damage may be cellulolytic. The objective of this study was to detect the cellulolytic fungi from deteriorated old Chinese manuscripts from Central Library Universitas Indonesia. The cellulolytic assay used microcrystalline cellulose (MCC), a nearly pure cellulose and water insoluble form, and carboxymethyl cellulose (CMC), a water-soluble form. A total of 25 fungal strains from deteriorated old Chinese manuscripts were tested for the possibility to degrade MCC and CMC. The strains were grown in a single point inoculation on modified Czapek Dox Agar plates without carbon source, and 1% (w/v) MCC or 1 % (w/v) CMC was added as a sole carbon source. The results showed that fungal strains with cellulolytic activity formed halo formation after dyeing with Congo red. A total of 18 strains (72%) were able to use CMC, and 14 fungal strains (56%) were able to use MCC. Thirteen strains (52%) were able to use both MCC and CMC, and they were members of six genera (Anthostomella, Aspergillus, Cladosporium, Flavomyces, Penicillium, and Sarocladium). Six strains (24%) were not able to use both MCC and CMC. It was suggested that many of the fungal strains in this study employed the synergistic reaction of both exoglucanase and endoglucanase to degrade MCC, and endoglucanase (carboxymethylcellulase) to degrade CMC. The present study showed that the potential cause of the deterioration of old Chinese manuscripts were the cellulolytic fungal strains from the manuscripts.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

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