Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

F. A. Rubin; P. D. McWhirter; N. H. Punjabi; E. Lane; Pratiwi Pudjilestari Sudarmono; S. P. Pulungsih; M. Lesmana; S. Kumala; D. J. Kopecko; S. L. Hoffman

doi:10.1128/jcm.27.5.1112-1114.1989

Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

F. A. Rubin, P. D. McWhirter, N. H. Punjabi, E. Lane, Pratiwi Pudjilestari Sudarmono, S. P. Pulungsih, M. Lesmana, S. Kumala, D. J. Kopecko, S. L. Hoffman

Department of Anesthesiology and Intensive Care

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.

Original language	English
Pages (from-to)	1112-1114
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	27
Issue number	5
DOIs	https://doi.org/10.1128/jcm.27.5.1112-1114.1989
Publication status	Published - 1989

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10.1128/jcm.27.5.1112-1114.1989

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title = "Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever",

abstract = "A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.",

author = "Rubin, {F. A.} and McWhirter, {P. D.} and Punjabi, {N. H.} and E. Lane and Sudarmono, {Pratiwi Pudjilestari} and Pulungsih, {S. P.} and M. Lesmana and S. Kumala and Kopecko, {D. J.} and Hoffman, {S. L.}",

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doi = "10.1128/jcm.27.5.1112-1114.1989",

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T1 - Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

AU - Rubin, F. A.

AU - McWhirter, P. D.

AU - Punjabi, N. H.

AU - Lane, E.

AU - Sudarmono, Pratiwi Pudjilestari

AU - Pulungsih, S. P.

AU - Lesmana, M.

AU - Kumala, S.

AU - Kopecko, D. J.

AU - Hoffman, S. L.

PY - 1989

Y1 - 1989

N2 - A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.

AB - A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.

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U2 - 10.1128/jcm.27.5.1112-1114.1989

DO - 10.1128/jcm.27.5.1112-1114.1989

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C2 - 2745686

AN - SCOPUS:0024538791

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever

Abstract

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