Uptake mechanisms of cell-internalizing nucleic acid aptamers for applications as pharmacological agents

Samira Husen Alamudi, Michiko Kimoto, Ichiro Hirao

Research output: Contribution to journalReview articlepeer-review

8 Citations (Scopus)

Abstract

Nucleic acid aptamers, also regarded as chemical antibodies, show potential as targeted therapeutic and delivery agents since they possess unique advantages over antibodies. Generated by an iterative selection and amplification process from oligonucleotide libraries using cultured cells, the aptamers bind to their target molecules expressed on the cell surface. Excitingly, most aptamers also demonstrate a cell-internalizing property in native living cells, allowing them to directly enter the cellsviaendocytosis depending on the target. In this review, we discuss selection methods in generating cell-internalizing aptamersviaa cell-based selection process, along with their challenges and optimization strategies. We highlight the cellular uptake routes adopted by the aptamers and also their intracellular fate after the uptake, to give an overview of their mechanism of action for applications as promising pharmacological agents.

Original languageEnglish
Pages (from-to)1640-1649
Number of pages10
JournalRSC Medicinal Chemistry
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 2021

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