TY - JOUR
T1 - Umbilical cord derived mesenchymal stem cell proliferation in various platelet rich plasma and xeno-material containing medium
AU - Budiyanti, Ecie
AU - Liem, Isabella Kurnia
AU - Adiwinata, Jeanne
AU - Wulandari, Dewi
AU - Jamaan, Taufik
AU - Sumapradja, Kanadi
N1 - Publisher Copyright:
© JK Welfare & Pharmascope Foundation.
PY - 2015/3/30
Y1 - 2015/3/30
N2 - Previous studies used fetal bovine serum (FBS) containing medium for umbilical cord derived mesenchymal stem cell culture. Xenoproteins in FBS may be incorporated into the cultured cells and cause immune rejection, when the cells are used in patients. Therefore, the aim of this study was to compare propagation performance of umbilical cord derived stem cells that were cultured in various platelet rich plasma (PRP) containing media and FBS derivate containing commercial medium.This study was an in vitro analytical study on multiple harvest explant culture derived stem cells, which compared the stem cell population doubling time (PDT) of passage 1-3 cultures in human AB PRP containing media (low glucose and high glucose DMEM, and αMEM) compared to xenopretein containing commercial medium, MesenCult®. Overall, the lowest PDT was achieved by αMEM-PRP, and PDT in DMEM LG-PRP and αMEM PRP was comparable to those of MesenCult®. Features of cluster of differentiation (CD) expressions in P-1 suggest that the umbilical cord derived stem cells obtained from multiple harvest explant cul-ture were not homogenous for MSCs. However, MSCs tended to become more homogenous with passages, espe-cially for cells that were cultured in MesenCult®, followed by those cultured in αMEM-PRP. In conclusion, the best propagation performance of umbilical cord derived stem cells passage 1-3 cultures was achieved in PRP containing αMEM.
AB - Previous studies used fetal bovine serum (FBS) containing medium for umbilical cord derived mesenchymal stem cell culture. Xenoproteins in FBS may be incorporated into the cultured cells and cause immune rejection, when the cells are used in patients. Therefore, the aim of this study was to compare propagation performance of umbilical cord derived stem cells that were cultured in various platelet rich plasma (PRP) containing media and FBS derivate containing commercial medium.This study was an in vitro analytical study on multiple harvest explant culture derived stem cells, which compared the stem cell population doubling time (PDT) of passage 1-3 cultures in human AB PRP containing media (low glucose and high glucose DMEM, and αMEM) compared to xenopretein containing commercial medium, MesenCult®. Overall, the lowest PDT was achieved by αMEM-PRP, and PDT in DMEM LG-PRP and αMEM PRP was comparable to those of MesenCult®. Features of cluster of differentiation (CD) expressions in P-1 suggest that the umbilical cord derived stem cells obtained from multiple harvest explant cul-ture were not homogenous for MSCs. However, MSCs tended to become more homogenous with passages, espe-cially for cells that were cultured in MesenCult®, followed by those cultured in αMEM-PRP. In conclusion, the best propagation performance of umbilical cord derived stem cells passage 1-3 cultures was achieved in PRP containing αMEM.
KW - Flow cytometry
KW - Mesenchymal stem cell
KW - Platelet rich plasma
KW - Population doubling time
KW - Umbilical cord
UR - http://www.scopus.com/inward/record.url?scp=84935924184&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84935924184
SN - 0975-7538
VL - 6
SP - 7
EP - 13
JO - International Journal of Research in Pharmaceutical Sciences
JF - International Journal of Research in Pharmaceutical Sciences
IS - 1
ER -