TY - JOUR
T1 - Ultrastructure of Javaen barb fish Systomus orphoides Valenciennes, 1842 spermatozoa (Cypriniformes: Cyprinidae) by electron microscopes
AU - Hilia, Sri Widiyanti Rahayu
AU - Abinawanto, null
AU - Dwiranti, Astari
AU - Bowolaksono, Anom
AU - Lestari, Retno
AU - Fadhilah, null
AU - Kristanto, Anang Hari
AU - Ohmido, Nobuko
N1 - Funding Information:
We are thankful to the Directorate of Research and Community Services at University of Indonesia for allowing us to use the Integrated Laboratory and Research Center. We are also grateful to Prof. Kiichi Fukui at the Graduate School of Pharmaceutical Sciences, Osaka University, and Prof. Kaoru Mitsuoka at UHVEM Center, Osaka University for their support on ultrastructure analysis. The author is grateful to the Ministry of Research and Technology of Indonesia through the Master and Doctoral Education program for Superior Bachelors (PMDSU) 2022. This research was funded based on the contract number, PMDSU: NKB‐825/UN2.RST/HKP.05.00/2022 and PKPI: 085.9/E4.4/KU/2022.
Publisher Copyright:
© 2023 Wiley Periodicals LLC.
PY - 2023/11
Y1 - 2023/11
N2 - Javaen barb fish Systomus orphoides Valenciennes, 1842 (Cypriniformes: Cyprinidae) is a freshwater fish whose population is declining and threatened with extinction. In this study, the ultrastructure of spermatozoa of Javaen barb fish (S. orphoides) was studied using transmission and scanning electron microscopy. The spermatozoa of S. orphoides are relatively simple cells composed of a spherical head, a short midpiece, and a flagellum, as in most Cyprinidae. The ultrastructure is characterized by the absence of acrosome, the total length of spermatozoa is 27.16 ± 4.5 μm, and the head has spherical with a length of 1.84 ± 0.10 μm and width of 1.55 ± 0.15 μm containing a nucleus, midpiece region containing the proximal and distal centrioles and mitochondria. Two or three mitochondria surrounding the axoneme (with a 9 + 2 microtubular pattern). Ultrastructural analyses by SEM and TEM of Javaen barb fish spermatozoa cells are very consistent with those of Cyprinidae. This study provides the ultrastructure information of S. orphoides spermatozoa in the Cyprinidae family this research could be useful in increasing reproductive efficiency and further prevent the extinction of this species.
AB - Javaen barb fish Systomus orphoides Valenciennes, 1842 (Cypriniformes: Cyprinidae) is a freshwater fish whose population is declining and threatened with extinction. In this study, the ultrastructure of spermatozoa of Javaen barb fish (S. orphoides) was studied using transmission and scanning electron microscopy. The spermatozoa of S. orphoides are relatively simple cells composed of a spherical head, a short midpiece, and a flagellum, as in most Cyprinidae. The ultrastructure is characterized by the absence of acrosome, the total length of spermatozoa is 27.16 ± 4.5 μm, and the head has spherical with a length of 1.84 ± 0.10 μm and width of 1.55 ± 0.15 μm containing a nucleus, midpiece region containing the proximal and distal centrioles and mitochondria. Two or three mitochondria surrounding the axoneme (with a 9 + 2 microtubular pattern). Ultrastructural analyses by SEM and TEM of Javaen barb fish spermatozoa cells are very consistent with those of Cyprinidae. This study provides the ultrastructure information of S. orphoides spermatozoa in the Cyprinidae family this research could be useful in increasing reproductive efficiency and further prevent the extinction of this species.
KW - Javaen barb fish (Systomus orphoides)
KW - morphology spermatozoa
KW - scanning electron microscope
KW - transmission electron microscope
KW - ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=85158826509&partnerID=8YFLogxK
U2 - 10.1002/jemt.24341
DO - 10.1002/jemt.24341
M3 - Article
AN - SCOPUS:85158826509
SN - 1059-910X
VL - 86
SP - 1411
EP - 1415
JO - Microscopy Research and Technique
JF - Microscopy Research and Technique
IS - 11
ER -