Hyperuricemia is a condition that uric acid levels increased and cause accumulated uric acid crystals in the tissues. Xanthine oxidase is an enzyme which catalyze the oxidation of hypoxanthine into xanthine and into uric acid. Therefore, the inhibition of xanthine oxidase will reduce ammount of uric acid. The purpose of this study was to determine xanthine oxidase inhibition activity and to identify chemical constituent of rrind rambutan (Nephelium lappaceum Linn.). Rind of Rambutan was extracted using maceration methods, based on polarity the solvent are n-hexane, ethyl acetate and methanol. The test of inhibition xanthine oxidase activity used a spectrophotometer at λ = 274.79 nm, pH 7.8, substrate concentration of xanthine 0.15 mM and incubation temperature of 30°C. Allopurinol as positive control had C50 0.15 μg/mL. The result showed that methanol extract of rind rambutan had the highest inhibition with IC50 3.71 μg/mL. Phytochemical screening showed that the most active extract methanol of rind rambutan contain flavonoids, saponins, tannins and terpenoids.