Uji Penghambatan Aktivitas alfa-glukosidase Ekstrak dan Fraksi Daun Antidesma neurocarpum Miq.

Rieka Widihasputri, Berna Elya, Azizahwati

Research output: Contribution to journalArticle

Abstract

Diabetes mellitus is a metabolic dysfunction disease showed by hyperglicemia and abnormality of carbohydrates, fats, and proteins metabolism. It is known can be cured with a-glucosidase inhibitor. Previous experiment reported that methanol extract from leaves of Antidesma neurocarpum Miq. has a strong inhibitory activity of a-glucosidase (IC50 = 2,18 mikrogram/mL). The aim of this experiment was to know about the most active extract and fraction of inhibitory activity of a-glycosidase and also the chemical compounds from those most active extract and fraction. Extraction is done by using multilevel maceration (n-hexane, ethyl acetate and methanol). Inhibitory activity of a-glucosidase tested with

microplate reader at 405 nm wavelength. As standard, we used carbose (IC50=38.37 gg/mL). Methanol extract has the strongest inhibitory activity of a-glucosidase as shown the highest inhibitor percentage (89.53%). It is identified the chemical compounds and

fracinationed by flash column chromatography. Fractination produced 15 combined fractions. Combined fractions which more than 200 mg tested to know their inhibitory activity of a-glucosidase. Result showed that the most active fraction is the 8th (IC50=40.77 gg/mL) with a competitive inhibitor mechanism to a-glycosidase. Chemical compounds that is found in the methanol extract and 8th fraction of Antidesma neurocarpum Miq. leaves are flavonoids, tannins, glycosides, fenol and alkaloids.
Original languageIndonesian
JournalPharmaceutical Sciences and Research
Volume9
Issue number1
DOIs
Publication statusPublished - 2012

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