TY - GEN
T1 - Tyrosinase inhibitory activity of Garcinia xanthochymus fruit pericarp extract
AU - Ambarwati, Neneng Siti S.
AU - Elya, Berna
AU - Desmiaty, Yesi
AU - Atmanto, Dwi
AU - Ahmad, Islamudin
N1 - Funding Information:
The authors acknowledge to gratitude The Directorate of Research and Community Service, Ministry of Research, Technology and Higher Education of the Republic of Indonesia, which has funded this research on a primary research grant in 2020 with the assignment agreement number: 26 / SP2H / DRPM / LPPM / III / 2020.
Publisher Copyright:
© 2021 Author(s).
PY - 2021/4/2
Y1 - 2021/4/2
N2 - The tyrosinase is the main enzyme involved in melanin biosynthesis. The amount of melanin produced by melanocyte cells will affect a person's skin color. Therefore, the body's skin needs a compound that can inhibit the work of the enzyme tyrosinase. The purpose of this study was to determine the potential of G. xanthochymus fruit extracts as a whitening agent. The dried sample was continuously macerated using n-hexane and methanol. Furthermore, each extract's tyrosinase enzyme inhibitory activity was measured using a microplate reader 96 well spectrophotometers at 490 nm and a substrate of 3,4-dihydroxy-L-phenylalanine, L-D OPA. The results showed that the results of the Inhibitory Activity test at a concentration of 100 ppm from each extract were 35.41±11.10% (hexane extract), and 28.83 ± 6.77% (methanol extract). Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm. The conclusion of this research is that G. xanthochymus pericarp hexane and methanol extract have the potential as skin lightening with hexane extract results better than methanol extract, with inhibitory activity of tyrosinase enzyme half of kojic acid.
AB - The tyrosinase is the main enzyme involved in melanin biosynthesis. The amount of melanin produced by melanocyte cells will affect a person's skin color. Therefore, the body's skin needs a compound that can inhibit the work of the enzyme tyrosinase. The purpose of this study was to determine the potential of G. xanthochymus fruit extracts as a whitening agent. The dried sample was continuously macerated using n-hexane and methanol. Furthermore, each extract's tyrosinase enzyme inhibitory activity was measured using a microplate reader 96 well spectrophotometers at 490 nm and a substrate of 3,4-dihydroxy-L-phenylalanine, L-D OPA. The results showed that the results of the Inhibitory Activity test at a concentration of 100 ppm from each extract were 35.41±11.10% (hexane extract), and 28.83 ± 6.77% (methanol extract). Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm. The conclusion of this research is that G. xanthochymus pericarp hexane and methanol extract have the potential as skin lightening with hexane extract results better than methanol extract, with inhibitory activity of tyrosinase enzyme half of kojic acid.
UR - http://www.scopus.com/inward/record.url?scp=85103851077&partnerID=8YFLogxK
U2 - 10.1063/5.0041813
DO - 10.1063/5.0041813
M3 - Conference contribution
AN - SCOPUS:85103851077
T3 - AIP Conference Proceedings
BT - 2nd Science and Mathematics International Conference, SMIC 2020
A2 - Meiliasari, Meiliasari
A2 - Rahmawati, Yuli
A2 - Delina, Mutia
A2 - Fitriani, Ella
PB - American Institute of Physics Inc.
T2 - 2nd Science and Mathematics International Conference: Transforming Research and Education of Science and Mathematics in the Digital Age, SMIC 2020
Y2 - 8 August 2020 through 9 August 2020
ER -