Tyrosinase inhibitory activity of Garcinia daedalanthera Pierre

N. S.S. Ambarwati, B. Elya, Y. Desmiaty, I. Ahmad

Research output: Contribution to journalConference articlepeer-review

Abstract

The tyrosinase enzyme catalyzes the first stage in two reactions in the synthesis of melanin (the hydroxylation of l-tyrosinase becomes 3,4-dihydroxyphenylalanine (l- DOPA) and oxidation from L-dopa to dopaquinone). Hyperpigmentation in human skin is a general phenomenon that is not desirable. Researchers were encouraged to identify potential new tyrosinase inhibitors for cosmetics, especially anti-hyperpigmentation. This study purposed to determine the inhibitory activity of the tyrosinase enzyme from Garcinia daedalanthera Pierre leaves. The extract was obtained by maceration successively method. The anti-tyrosinase assay used the spectrophotometric method at 490 nm. The enzyme used tyrosinase from mushrooms lyophilized powder (Sigma), and the substrate used 3,4-dihydroxy-L-phenylalanine, L-DOPA (Sigma). The tyrosinase inhibitory assay results of 100 ppm (triplicate) showed the leaves extract, including ethyl acetate extract of 33.42 ± 5.98 %, hexane extract was 50.67 ± 0.47 %, and methanol extract of 50.68 ± 1.87 %, respectively. Moreover, the stem bark has activity as follows methanol extract of 43.76 ± 1.41 % and the ethyl acetate extract of 55.71 ± 2.80 %. Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm.

Original languageEnglish
Article number012019
JournalJournal of Physics: Conference Series
Volume1869
Issue number1
DOIs
Publication statusPublished - 15 Apr 2021
Event2nd Annual Conference of Science and Technology, ANCOSET 2020 - Malang, Virtual, Indonesia
Duration: 28 Nov 2020 → …

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