Transplantation of dental pulp stem cells in experimental bone defect

Endang W. Bachtiar, Dewi Fatma, Ahmad Aulia Yusuf, Rahmi Ulfiana

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2 Citations (Scopus)


This is preliminary study in order to investigate the effect of dental pulp stem cells (DPSCs) on bone regeneration in an animal model. New Zealand rabbits were used as animal model. The critical defect was created in femoral bone and transplantation of DPSCs applied into bone defect. A colorimetric assay was used to detect ALP level in rabbit's serum. Bone tissue regeneration was evaluated by histological analysis. In the 2nd week, the treated rabbit show increasing in the activity of ALP (157,925 ?U) compared to control rabbit (155,361 ?U). This increasing trend continues significantly in DPSCs rabbit (169.750 ?U) compared to control rabbit (160.406) after 4 weeks. Histological evaluation revealed that the amount of bone lamellae and osteocytes were filled the defect area of DPSCs treated rabbit. Conclusions: Transplantation of DPSCs accelerating bone regeneration by raising ALP level and forming new bone tissue.

Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalJournal of Biomimetics, Biomaterials and Biomedical Engineering
Publication statusPublished - 2017


  • Alkaline phosphatase
  • Bone regeneration
  • Dpscs


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