TY - JOUR
T1 - Transfersomal gel containing green tea (Camellia sinensis L. kuntze) leaves extract
T2 - Increasing in vitro penetration
AU - A, Effionora
AU - Utami, Tahmida Diazputri
AU - Ramadon, Delly
N1 - Publisher Copyright:
© 2017 The Authors.
PY - 2017
Y1 - 2017
N2 - Objective: The aim of this study was to increase penetration of epigallocatechin gallate (EGCG) from green tea leaves extract (Camellia sinensis L. Kuntz) through the skin by formulating the extract into a transfersomal gel (GT). Methods: Transfersomes were prepared by thin-layer hydration method, with different concentration of the extract that equivalent to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG. A transfersomes formula with good characteristics would be incorporated into a GT. A gel without transfersomes (GNT) was prepared as a control of comparison. Both gels were evaluated their physicochemical properties. An in vitro penetration test using Franz diffusion cell with the skin of female Sprague-Dawley rats was also performed. Results: The results showed that F1 had the best physicochemical properties. F1 had a spherical shape, Dmean volume at 107.82±0.44 nm, polydispersity index at 0.07±0.01, zeta potential at −40.3±0.10 mV, and entrapment efficiency at 63.16±0.65%. Cumulative amount of EGCG penetrated from GT and non-GT (GNT) was 1302.63±20.67 µg/cm2 and 414.86±4.40 µg/cm2, respectively (p<0.05). Flux penetration of GT and GNT was 57.594±0.91 µg/cm2 hrs and 36.144±1.22 µg/cm2. hrs, respectively. Conclusion: It can be concluded that GT could increase the in vitro penetration of EGCG from green tea leaves extract compared to non-transfersomal one.
AB - Objective: The aim of this study was to increase penetration of epigallocatechin gallate (EGCG) from green tea leaves extract (Camellia sinensis L. Kuntz) through the skin by formulating the extract into a transfersomal gel (GT). Methods: Transfersomes were prepared by thin-layer hydration method, with different concentration of the extract that equivalent to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG. A transfersomes formula with good characteristics would be incorporated into a GT. A gel without transfersomes (GNT) was prepared as a control of comparison. Both gels were evaluated their physicochemical properties. An in vitro penetration test using Franz diffusion cell with the skin of female Sprague-Dawley rats was also performed. Results: The results showed that F1 had the best physicochemical properties. F1 had a spherical shape, Dmean volume at 107.82±0.44 nm, polydispersity index at 0.07±0.01, zeta potential at −40.3±0.10 mV, and entrapment efficiency at 63.16±0.65%. Cumulative amount of EGCG penetrated from GT and non-GT (GNT) was 1302.63±20.67 µg/cm2 and 414.86±4.40 µg/cm2, respectively (p<0.05). Flux penetration of GT and GNT was 57.594±0.91 µg/cm2 hrs and 36.144±1.22 µg/cm2. hrs, respectively. Conclusion: It can be concluded that GT could increase the in vitro penetration of EGCG from green tea leaves extract compared to non-transfersomal one.
KW - Epigallocatechin gallate
KW - Green tea leaves extract
KW - In vitro penetration test
KW - Transfersomal gel
KW - Transfersomes
UR - http://www.scopus.com/inward/record.url?scp=85026817424&partnerID=8YFLogxK
U2 - 10.22159/ajpcr.2017.v10i8.19124
DO - 10.22159/ajpcr.2017.v10i8.19124
M3 - Article
AN - SCOPUS:85026817424
SN - 0974-2441
VL - 10
SP - 294
EP - 298
JO - Asian Journal of Pharmaceutical and Clinical Research
JF - Asian Journal of Pharmaceutical and Clinical Research
IS - 8
ER -