Transfersomal gel containing green tea (Camellia sinensis L. kuntze) leaves extract: Increasing in vitro penetration

Effionora A, Tahmida Diazputri Utami, Delly Ramadon

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13 Citations (Scopus)


Objective: The aim of this study was to increase penetration of epigallocatechin gallate (EGCG) from green tea leaves extract (Camellia sinensis L. Kuntz) through the skin by formulating the extract into a transfersomal gel (GT). Methods: Transfersomes were prepared by thin-layer hydration method, with different concentration of the extract that equivalent to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG. A transfersomes formula with good characteristics would be incorporated into a GT. A gel without transfersomes (GNT) was prepared as a control of comparison. Both gels were evaluated their physicochemical properties. An in vitro penetration test using Franz diffusion cell with the skin of female Sprague-Dawley rats was also performed. Results: The results showed that F1 had the best physicochemical properties. F1 had a spherical shape, Dmean volume at 107.82±0.44 nm, polydispersity index at 0.07±0.01, zeta potential at −40.3±0.10 mV, and entrapment efficiency at 63.16±0.65%. Cumulative amount of EGCG penetrated from GT and non-GT (GNT) was 1302.63±20.67 µg/cm2 and 414.86±4.40 µg/cm2, respectively (p<0.05). Flux penetration of GT and GNT was 57.594±0.91 µg/cm2 hrs and 36.144±1.22 µg/cm2. hrs, respectively. Conclusion: It can be concluded that GT could increase the in vitro penetration of EGCG from green tea leaves extract compared to non-transfersomal one.

Original languageEnglish
Pages (from-to)294-298
Number of pages5
JournalAsian Journal of Pharmaceutical and Clinical Research
Issue number8
Publication statusPublished - 2017


  • Epigallocatechin gallate
  • Green tea leaves extract
  • In vitro penetration test
  • Transfersomal gel
  • Transfersomes


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