Transferrin gene cloning and sequencing analysis from Indonesian Nile Tilapia (Oreochromis niloticus)

Rizky Priambodo, Irvan Faizal, Abinawanto, Wibowo Mangunwardoyo, Anom Bowolaksono

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Some fish have ability to live in the wide salinity range. Nile Tilapia (Oreochromis niloticus) is one example of fish that can tolerate the changes of the salinity in the water. Transferrin is one of many factors that makes Nile Tilapia fish can live in very wide salinity range. In Indonesia, Nile Tilapia usually lived in freshwater area. The purpose of this research is to clone and sequencing transferrin gene in Indonesian Nile Tilapia fish (Oreochromis niloticus). The subject of this research are three variants of Nile Tilapia fish which represent the population of Nile Tilapia fish in Indonesia, which is GESIT, Red Nifi, and Selfam. The DNA was taken from caudal fin and extracted by Phenol Chloroform Isoamyl-alcohol (PCI) method. Transferrin gene was amplified using TRF F2 primer (TTGACAACTATRAMRCCTGC TYCC) and TRF R1 primer (CAGCSGTAAACACCTGACCT) which resulted 800 bp DNA fragment. The DNA fragment from the agarose gel is then cut and purified. Transferrin gene fragment was cloned using pGEM ® -T easy plasmid and was transformed into E.coli DH5α using heat shocked method. The cells which have transferrin gene were extracted and then used to do the sequencing. The result of this research was the transferrin gene from three variants can be successfully cloned into E.coli DH5α and also successfully been sequenced. Those sequences information already submitted in the gene bank with accession code KT804150.1 for tilapia strain GESIT, KT804151.1 for strain Red Nifi, and KT804152.1 for strain Selfam. The sequence information of transferrin gene from three variants of Indonesian Nile Tilapia will be useful for the next research to develop the salinity tolerant of Nile Tilapia fish in Indonesia.

Original languageEnglish
Title of host publicationInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
Subtitle of host publicationProceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
EditorsTerry Mart, Djoko Triyono
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413764
DOIs
Publication statusPublished - 19 Apr 2016
Event1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
Duration: 3 Nov 20154 Nov 2015

Publication series

NameAIP Conference Proceedings
Volume1729
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
CountryIndonesia
CityDepok
Period3/11/154/11/15

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