@inproceedings{edea0ae0525a451898fcdfa83159101d,
title = "Toxicity and biocompatibility profile of 3D bone scaffold developed by Universitas Indonesia: A preliminary study",
abstract = "Scaffold as a biomaterial must fulfill some requirements to be safely implanted to the human body. Toxicity and biocompatibility test are needed to evaluate scaffold material in mediating cell proliferation and differentiation, secreting extracelullar matrix and carrying biomolecular signals for cell communication. An in vitro study with mesenchymal stem cells consisted of direct contact test and indirect contact test using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay was conducted on 4 scaffolds made of poly-L-lactic acid (PLA), polyvinyl alcohol (PVA), and hydroxyapatite-poly (vinyl alcohol) composite. There were cells-substrate adhesion impairment, morphological changes, cell death and reduction in cell proliferation seen at 2nd and 6th day in most tested scaffold. Cell count result at day-6 showed proliferation inhibition of more than 50% cell death (inhibition value >50) in all tested scaffold. In MTT assay, two scaffolds were proven non-toxic. In conclusion, various scaffold materials showed different toxicity effect. The toxicity and biocompatibility profile in this study is a preliminary data for further research aiming to use those local-made scaffolds to fill human bone defect in various needs.",
keywords = "MTT assay, Toxicity, biocompatibility, mesenchymal stem cell, scaffold",
author = "Rahyussalim, {A. J.} and T. Kurniawati and D. Aprilya and R. Anggraini and Ghiska Ramahdita and Yudan Whulanza",
note = "Publisher Copyright: {\textcopyright} 2017 Author(s).; 1st International Symposium of Biomedical Engineering: Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2016 ; Conference date: 31-05-2016 Through 01-06-2016",
year = "2017",
month = feb,
day = "21",
doi = "10.1063/1.4976756",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Yudan Whulanza and Sugeng Supriadi and Muhamad Sahlan and Basari",
booktitle = "Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices",
address = "United States",
}