Toxicity analysis of RGD-chitosan from shrimp shell scaffold membranes toward human dental pulp cells

Objective: This study aimed to analyze RGD-Chitosan from Shrimp Shells’ Scaffolds’ (RCSSS) and CSSS membrane toxicity toward human dental pulp cells. Methods: Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determined using an MTT assay method. Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSS group 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%). Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells compared to CSSS membranes.