Neuropathic (nerve) pain is caused by damage, dysfunction or injury of nerves it will affect patient's quality of life because of chronicity and intensity. The pharmaceutical drug commonly used to treat neuropathic pain is antidepressants. However, Anti-depressant drug has dangerous side effects if consumed continuously. Herbs treatments use throughout the world for the treatment of neuropathic pain. Jamu Neuropathic Pain Reducer, which consists of aqueous multi herbs extract of nutmeg seed (Myristica fragrans), clove bud (Syzygium aromaticum) and red ginger rhizome (Zingiber officinale var rubrum) was extracted with variation temperature (60, 80, 100). This study aimed to figure out the total phenolic content (TPC) in the Jamu Neuropathic Pain Reducer used the Folin-Ciocalteu method. The highest TPC (33.58 ± 1.473mg GAE/g herb) happened at temperature 80. UPLC-QTOF-MS applied to identify the major bioactive compound in Jamu Neuropathic Pain Reducer extract used temperature extraction 80, analysis revealed the presence of compounds such as adenine, chlorogenic acid, miquelianin, quercitrin, 6-gingerol, myristicin, and eugenol. Jamu Neuropathic Pain Reducer reduced the immobility duration compared with the control negative (distilled water) (p < 0.001). The reductions of the immobility time were 8.3%, 36.4% and 30.1% for dosage 16.25 mL/kg, 32.5 mL/kg and 65 mL/kg, therefore, Jamu Neuropathic Pain Reducer had the potential to be an alternative as herbal medicine for neuropathic pain that less expensive and has minimum side effect.