Topical modified-olive oil (Dalethyne) for bacterial-infected wound: A study in the rats

Wawaimuli Arozal, Gregorius Bhaskara Wikanendra, Melva Louisa, Kayapan Satya Dharshan, Kusmardi Kusmardi, Ari Estuningtyas, Donna Savitry

Research output: Contribution to journalArticlepeer-review

Abstract

Open skin wound often leads to the infiltration of pathogens. Inadequate wound healing process might lead to systemic infections. Therefore, proper wound care is needed to prevent further possible complications. This study was aimed to investigate the effect of dalethyne administration on the wound healing process in open skin wounds infected by bacterias. We conducted an in vivo experiment in rats. The skin of the rats was cut with a 1-cm incision along the torso on the left, and the right side then was inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus epidermidis and Escherichia coli for 24-hour. Afterward, vehicle (glycerine) or dalethyne were administered to the wounds. Macroscopic and microscopic observations were done on Day 1 and Day 6 after treatment administration. Dalethyne application resulted in a significant wound closure rate compared with the vehicle only (glycerine) on skin wounds infected with Pseudomonas aeruginosa. No significant difference in wound closure rate for skin infected with Staphylococcus aureus, Staphylococcus epidermidis, and E. coli after topical administration with dalethyne versus vehicle (glycerine). However, microscopic observation showed a higher tendency of the healing process, as demonstrated by neutrophil and mononuclear infiltration in the wound areas. Dalethyne showed a beneficial healing rate in a rat model of bacteria-infected wounds. However, due to the short term observations in the present study, the more prolonged wound healing effect of dalethyne, still need to be investigated.

Original languageEnglish
Pages (from-to)1223-1228
Number of pages6
JournalJournal of International Dental and Medical Research
Volume13
Issue number3
Publication statusPublished - 2020

Keywords

  • Antibacterial
  • Dalethyne
  • Modified olive oil
  • Skin wound

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