Time-Resolved Thickness and Shape-Change Quantification using a Dual-Band Nanoplasmonic Ruler with Sub-Nanometer Resolution

Ferry Anggoro Ardy Nugroho, Dominika Świtlik, Antonius Armanious, Padraic O'Reilly, Iwan Darmadi, Sara Nilsson, Vladimir P. Zhdanov, Fredrik Höök, Tomasz J. Antosiewicz, Christoph Langhammer

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Time-resolved measurements of changes in the size and shape of nanobiological objects and layers are crucial to understand their properties and optimize their performance. Optical sensing is particularly attractive with high throughput and sensitivity, and label-free operation. However, most state-of-the-art solutions require intricate modeling or multiparameter measurements to disentangle conformational or thickness changes of biomolecular layers from complex interfacial refractive index variations. Here, we present a dual-band nanoplasmonic ruler comprising mixed arrays of plasmonic nanoparticles with spectrally separated resonance peaks. As electrodynamic simulations and model experiments show, the ruler enables real-time simultaneous measurements of thickness and refractive index variations in uniform and heterogeneous layers with sub-nanometer resolution. Additionally, nanostructure shape changes can be tracked, as demonstrated by quantifying the degree of lipid vesicle deformation at the critical coverage prior to rupture and supported lipid bilayer formation. In a broader context, the presented nanofabrication approach constitutes a generic route for multimodal nanoplasmonic optical sensing.

Original languageEnglish
Pages (from-to)15814-15826
Number of pages13
JournalACS Nano
Issue number10
Publication statusPublished - 25 Oct 2022


  • biomolecules
  • biosensors
  • conformation
  • nanoplasmonic sensors
  • nanorulers
  • supported lipid bilayer


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