TY - JOUR
T1 - Time-and dose-dependent effects of centela asiatica ethanolic extract encapsulated into chitosan nanoparticles on collagen III synthesis and proliferation in human dermal fibroblasts
AU - Yulianti, Linda
AU - Mardliyati, Etik
AU - Bramono, Kusmarinah
AU - Freisleben, Hans Joachim
N1 - Publisher Copyright:
© 2015 Dongjun Suh and Sungil Ham.
PY - 2016
Y1 - 2016
N2 - Natural aging process comes with the decline of skin cells renewal and collagen III production. Centella asiatica L. (CA) extracts are natural products which were shown to promote fibroblast proliferation during wound healing. Inthis study, we evaluated therapeutic potential of ethanolic CA extract (CAEE) when encapsulated into chitosan nanoparticles (CNP) by ionotropic gelation. Effects of CAEE-loaded CNP (CAEE+CNP) on the proliferation rate and collagen III synthesis of human dermal fibroblasts (HDF) were evaluated and compared against un-encapsulated CAEE. Proliferation rate of HDF was assessed with 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT assay), while ELISA was performed to evaluate Collagen III synthesis after 24, 48, or 72 hours incubation. For 24h incubation period, CAEE+CNP stimulates significantly greater HDF proliferation than CAEE alone. Moreover, in general we observed higher proliferation rate and collagen III synthesis on fibroblasts stimulated with CAEE+CNP as compared to CAEE alone. Thus, nano-encapsulation is important to maximize therapeutic potential of CAEE. In the future, the proposed formulation of CAEE-loaded CNP may be explored further as effective herbal agents in dermacosmetics for wound healing and anti-aging.
AB - Natural aging process comes with the decline of skin cells renewal and collagen III production. Centella asiatica L. (CA) extracts are natural products which were shown to promote fibroblast proliferation during wound healing. Inthis study, we evaluated therapeutic potential of ethanolic CA extract (CAEE) when encapsulated into chitosan nanoparticles (CNP) by ionotropic gelation. Effects of CAEE-loaded CNP (CAEE+CNP) on the proliferation rate and collagen III synthesis of human dermal fibroblasts (HDF) were evaluated and compared against un-encapsulated CAEE. Proliferation rate of HDF was assessed with 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT assay), while ELISA was performed to evaluate Collagen III synthesis after 24, 48, or 72 hours incubation. For 24h incubation period, CAEE+CNP stimulates significantly greater HDF proliferation than CAEE alone. Moreover, in general we observed higher proliferation rate and collagen III synthesis on fibroblasts stimulated with CAEE+CNP as compared to CAEE alone. Thus, nano-encapsulation is important to maximize therapeutic potential of CAEE. In the future, the proposed formulation of CAEE-loaded CNP may be explored further as effective herbal agents in dermacosmetics for wound healing and anti-aging.
KW - Centella asiatica extract
KW - Chitosan nanoparticles
KW - Collagen III
KW - Fibroblast
KW - MTT assay
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=84959537322&partnerID=8YFLogxK
U2 - 10.12988/ces.2016.512330
DO - 10.12988/ces.2016.512330
M3 - Article
AN - SCOPUS:84959537322
SN - 1313-6569
VL - 9
SP - 81
EP - 93
JO - Contemporary Engineering Sciences
JF - Contemporary Engineering Sciences
IS - 1-4
ER -