The use of single and mixed cultures of Bacillus cereus InaCC B284 and Pseudomonas aeruginosa InaCC B290 to degrade used-cooking oil

Nimas Ayu Rikmawati, Wibowo Mangunwardoyo, Hanies Ambarsari

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Used-cooking oil is a waste product after using cooking oil 3-4 times. The disposal of used cooking oil directly into waterways causes environmental pollution. Bacillus cereus and Pseudomonas aeruginosa are well known to be effective in degrading used-cooking oil. Biodegradation of used-cooking oil is needed to reduce the pollution created by the current disposal of oil. This study aimed to determine the capability of single and mixed cultures of B. cereus InaCC B284 and P. aeruginosa InaCC B290 to degrade used cooking oil. The biodegradation process was carried out using Bushnell Haas Broth (BHB) containing 25% (v/v) used cooking oil for 23 days at room temperature (27-30°C). Optical density (OD), pH, dissolved oxygen (DO), and fatty acid content were measured throughout the biodegradation process. Different bacterial treatments resulted in significant increases to the OD (p < 0.05) and significant decreases to the pH (p < 0.05) during biodegradation. Qualitative and quantitative fatty acid analysis performed by gas chromatography revealed that a single culture of B. cereus InaCC B284 was capable of degrading seven types of fatty acids, a single culture of P. aeruginosa InaCC B290 was capable of degrading four types of fatty acids, and mixed cultures were capable of degrading 11 types of fatty acids.

Original languageEnglish
Title of host publication8th Annual Basic Science International Conference
Subtitle of host publicationCoverage of Basic Sciences toward the World's Sustainability Challenges
EditorsCorina Karim, Rodliyati Azrianingsih, Mauludi Ariesto Pamungkas, Yoga Dwi Jatmiko, Anna Safitri
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417397
DOIs
Publication statusPublished - 17 Oct 2018
Event8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 - Malang, East Java, Indonesia
Duration: 6 Mar 20187 Mar 2018

Publication series

NameAIP Conference Proceedings
Volume2021
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
CountryIndonesia
CityMalang, East Java
Period6/03/187/03/18

Keywords

  • Bacillus cereus InaCC B284
  • Pseudomonas aeruginosa InaCC B290
  • biodegradation
  • used cooking oil

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