TY - GEN
T1 - The use of single and mixed cultures of Bacillus cereus InaCC B284 and Pseudomonas aeruginosa InaCC B290 to degrade used-cooking oil
AU - Rikmawati, Nimas Ayu
AU - Mangunwardoyo, Wibowo
AU - Ambarsari, Hanies
PY - 2018/10/17
Y1 - 2018/10/17
N2 - Used-cooking oil is a waste product after using cooking oil 3-4 times. The disposal of used cooking oil directly into waterways causes environmental pollution. Bacillus cereus and Pseudomonas aeruginosa are well known to be effective in degrading used-cooking oil. Biodegradation of used-cooking oil is needed to reduce the pollution created by the current disposal of oil. This study aimed to determine the capability of single and mixed cultures of B. cereus InaCC B284 and P. aeruginosa InaCC B290 to degrade used cooking oil. The biodegradation process was carried out using Bushnell Haas Broth (BHB) containing 25% (v/v) used cooking oil for 23 days at room temperature (27-30°C). Optical density (OD), pH, dissolved oxygen (DO), and fatty acid content were measured throughout the biodegradation process. Different bacterial treatments resulted in significant increases to the OD (p < 0.05) and significant decreases to the pH (p < 0.05) during biodegradation. Qualitative and quantitative fatty acid analysis performed by gas chromatography revealed that a single culture of B. cereus InaCC B284 was capable of degrading seven types of fatty acids, a single culture of P. aeruginosa InaCC B290 was capable of degrading four types of fatty acids, and mixed cultures were capable of degrading 11 types of fatty acids.
AB - Used-cooking oil is a waste product after using cooking oil 3-4 times. The disposal of used cooking oil directly into waterways causes environmental pollution. Bacillus cereus and Pseudomonas aeruginosa are well known to be effective in degrading used-cooking oil. Biodegradation of used-cooking oil is needed to reduce the pollution created by the current disposal of oil. This study aimed to determine the capability of single and mixed cultures of B. cereus InaCC B284 and P. aeruginosa InaCC B290 to degrade used cooking oil. The biodegradation process was carried out using Bushnell Haas Broth (BHB) containing 25% (v/v) used cooking oil for 23 days at room temperature (27-30°C). Optical density (OD), pH, dissolved oxygen (DO), and fatty acid content were measured throughout the biodegradation process. Different bacterial treatments resulted in significant increases to the OD (p < 0.05) and significant decreases to the pH (p < 0.05) during biodegradation. Qualitative and quantitative fatty acid analysis performed by gas chromatography revealed that a single culture of B. cereus InaCC B284 was capable of degrading seven types of fatty acids, a single culture of P. aeruginosa InaCC B290 was capable of degrading four types of fatty acids, and mixed cultures were capable of degrading 11 types of fatty acids.
KW - Bacillus cereus InaCC B284
KW - Pseudomonas aeruginosa InaCC B290
KW - biodegradation
KW - used cooking oil
UR - http://www.scopus.com/inward/record.url?scp=85056163359&partnerID=8YFLogxK
U2 - 10.1063/1.5062734
DO - 10.1063/1.5062734
M3 - Conference contribution
AN - SCOPUS:85056163359
T3 - AIP Conference Proceedings
BT - 8th Annual Basic Science International Conference
A2 - Karim, Corina
A2 - Azrianingsih, Rodliyati
A2 - Pamungkas, Mauludi Ariesto
A2 - Jatmiko, Yoga Dwi
A2 - Safitri, Anna
PB - American Institute of Physics Inc.
T2 - 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
Y2 - 6 March 2018 through 7 March 2018
ER -