TY - JOUR
T1 - The use of salivary specimen for COVID-19 detection using RT-PCR assay
T2 - a systematic review
AU - Budiyatno, Kevin Chrisanta
AU - Wibowo, Adik
AU - Riwu, Samuel Lay
N1 - Publisher Copyright:
© 2022, Intelektual Pustaka Media Utama. All rights reserved.
PY - 2022/6
Y1 - 2022/6
N2 - As coronavirus disease 2019 (COVID-19) cases arose globally, active case finding by performing throat swab test proposed high risk for the healthcare workers. Saliva had recently been reported to show positive detection means for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and proposed advantages of self-collection, less requirement of transport media, and reduced nosocomial transmission risk. However, support evidence regarding its diagnostic value was still lacking and varied widely in specimen collection method. This systematic review aimed to assess the diagnostic value of salivary specimens (SS) for COVID-19 detection using reverse transcription polymerase chain reaction (RT-PCR) assay compared with throat swab specimens (TSS), while putting into consideration confounders such as patients’ initial condition, specimen collection method, and transport media used. Six databases were used for identifying relevant studies. Final search yielded 19 eligible studies which was reviewed based on the major outcome: diagnostic agreement, sensitivity & specificity, and viral load comparison. The use of SS as an alternative to TSS showed to be promising although specimen collection method needed to be standardized. SS was comparable to TSS in detecting COVID-19 using RT-PCR assay, especially in symptomatic or confirmed cases. More Randomized controlled trials (RCTs) were still needed to clearly demonstrate the ability of SS to capture asymptomatic cases in the setting of mass surveillance, where patients would self-collect the specimen at ease.
AB - As coronavirus disease 2019 (COVID-19) cases arose globally, active case finding by performing throat swab test proposed high risk for the healthcare workers. Saliva had recently been reported to show positive detection means for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and proposed advantages of self-collection, less requirement of transport media, and reduced nosocomial transmission risk. However, support evidence regarding its diagnostic value was still lacking and varied widely in specimen collection method. This systematic review aimed to assess the diagnostic value of salivary specimens (SS) for COVID-19 detection using reverse transcription polymerase chain reaction (RT-PCR) assay compared with throat swab specimens (TSS), while putting into consideration confounders such as patients’ initial condition, specimen collection method, and transport media used. Six databases were used for identifying relevant studies. Final search yielded 19 eligible studies which was reviewed based on the major outcome: diagnostic agreement, sensitivity & specificity, and viral load comparison. The use of SS as an alternative to TSS showed to be promising although specimen collection method needed to be standardized. SS was comparable to TSS in detecting COVID-19 using RT-PCR assay, especially in symptomatic or confirmed cases. More Randomized controlled trials (RCTs) were still needed to clearly demonstrate the ability of SS to capture asymptomatic cases in the setting of mass surveillance, where patients would self-collect the specimen at ease.
KW - COVID-19
KW - Detection
KW - Diagnostic value
KW - RT-PCR
KW - Salivary specimen
UR - http://www.scopus.com/inward/record.url?scp=85126961358&partnerID=8YFLogxK
U2 - 10.11591/ijphs.v11i2.21173
DO - 10.11591/ijphs.v11i2.21173
M3 - Article
AN - SCOPUS:85126961358
VL - 11
SP - 646
EP - 653
JO - International Journal of Public Health Science
JF - International Journal of Public Health Science
SN - 2252-8806
IS - 2
ER -