The Use of Chitosan Nanoparticles for Delivery of CpG ODN in Treatment of Allergic Balb/C Mice

Febriana Catur Iswanti, Qarina Hasyala Putri, Ani Retno Prijanti, Samsuridjal Djauzi, Mohamad Sadikin, Arief Budi Witarto, Tomohiko Yamazaki

Research output: Contribution to journalArticlepeer-review

Abstract

Background: This study aims to prepare high stability chitosan nanoparticles (CNP) and examine the ability of CNP in CpG-ODN delivery when treating allergic mice model. Methods: Preparation and characterization of CNP were performed by ionic gelation, dynamic light scattering, and zeta sizer. The CNP cytotoxicity and activation ability of CpG ODN delivered with CNP were tested using a cell counting kit-8 and Quanti blue method. Allergic mice were injected intraperitoneal with 10 ug ovalbumin on day 0 and 7, and then treated with intranasal CpG ODN/CpG ODN, delivered with CNP/CNP, on the third week three times per week for three weeks. The ELISA method measured cytokine and IgE profiles in the allergic mice’s plasma and spleen. Results: CNP results have sizes 27.73 nm±3.67 dan 188.23 nm±53.47, spherical in shape and nontoxic, and did not alter the NF-κB activation of CpG ODN in RAW-blue cells. The application of CpG ODN delivered by chitosan nanoparticles shows no statistical difference between groups of IFN-γ, IL-10, and IL-13 in Balb/c mice’s plasma and spleen, in contrast with IgE level.

Original languageEnglish
Pages (from-to)600-613
Number of pages14
JournalReports of Biochemistry and Molecular Biology
Volume11
Issue number4
Publication statusPublished - 2023

Keywords

  • Allergy
  • Chitosan nanoparticle
  • CpG ODN
  • Immunotherapy
  • Mice spleen

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