The use of a needle-free injector for DNA vaccination in BALB/c mice

Fera Ibrahim, Silvia T. Widyaningtyas, Ekawati Betty Pratiwi, Budiman Bela

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The capability of DNA vaccine to transfect cells determines the success of DNA vaccine, but there are some natural obstacles that reduce the efficiency of DNA delivery into cells. One way to overcome this problem is through the use of a delivery systems such as needle free injectors (NFIT). In the present study, we evaluated 2 kinds of NFITs, Injex and Twin-Jector EZII, and as control we use hypodermic syringes. The path of injection and the capability of NFITs to deliver DNA into cells were observed by using India ink and plasmid coding enhanced green fluorescent protein (pCDNA eGFP) respectively. A mass of 50 μg pcDNA3.1-eGFP in 50 μL of PBS and India ink were injected into the mouse thigh using these three delivery systems. Two days post-injection, histological slides were prepared from the thigh muscle and the expression of eGFP was observed by confocal microscopy. The results show that all the delivery systems used in the present study were able to deliver India ink and pcDNA3.1-eGFP to the thigh muscle. There was a difference in the spread of Indian ink produced 3 kinds of delivery systems used in this study. Furthermore, there was also a difference in the distribution of eGFP expression cells, that corelated with the pattern of the path /spread of fluid produced by the three delivery systems. The number and intensity cell expressing eGFP was intense in mice injecting with hypodermic syring compare to Injex and Twin-Jector EZII. - The results seen with India ink support the eGFP expression results. In conclusion, needle free injection can be used to deliver DNA into BALB/c muscle, but less efficient compared to hypodermic syringe.

Original languageEnglish
Title of host publicationProceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
Subtitle of host publicationTowards Innovative Research and Cross-Disciplinary Collaborations
EditorsNaji Arafat Mahat, Roswanira Ab. Wahab, Fahrul Zaman Huyop, Aemi Syazwani Abdul Keyon, Nursyafreena Binti Attan, Sheela Chandren, Ida Bagus Wayan Gunam
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419001
DOIs
Publication statusPublished - 6 Sep 2019
Event2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019 - Bali, Indonesia
Duration: 11 Apr 201912 Apr 2019

Publication series

NameAIP Conference Proceedings
Volume2155
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
CountryIndonesia
CityBali
Period11/04/1912/04/19

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  • Cite this

    Ibrahim, F., Widyaningtyas, S. T., Pratiwi, E. B., & Bela, B. (2019). The use of a needle-free injector for DNA vaccination in BALB/c mice. In N. A. Mahat, R. A. Wahab, F. Z. Huyop, A. S. A. Keyon, N. B. Attan, S. Chandren, & I. B. W. Gunam (Eds.), Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations [020033] (AIP Conference Proceedings; Vol. 2155). American Institute of Physics Inc.. https://doi.org/10.1063/1.5125537