TY - GEN
T1 - The use of a needle-free injector for DNA vaccination in BALB/c mice
AU - Ibrahim, Fera
AU - Widyaningtyas, Silvia T.
AU - Pratiwi, Ekawati Betty
AU - Bela, Budiman
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/9/6
Y1 - 2019/9/6
N2 - The capability of DNA vaccine to transfect cells determines the success of DNA vaccine, but there are some natural obstacles that reduce the efficiency of DNA delivery into cells. One way to overcome this problem is through the use of a delivery systems such as needle free injectors (NFIT). In the present study, we evaluated 2 kinds of NFITs, Injex and Twin-Jector EZII, and as control we use hypodermic syringes. The path of injection and the capability of NFITs to deliver DNA into cells were observed by using India ink and plasmid coding enhanced green fluorescent protein (pCDNA eGFP) respectively. A mass of 50 μg pcDNA3.1-eGFP in 50 μL of PBS and India ink were injected into the mouse thigh using these three delivery systems. Two days post-injection, histological slides were prepared from the thigh muscle and the expression of eGFP was observed by confocal microscopy. The results show that all the delivery systems used in the present study were able to deliver India ink and pcDNA3.1-eGFP to the thigh muscle. There was a difference in the spread of Indian ink produced 3 kinds of delivery systems used in this study. Furthermore, there was also a difference in the distribution of eGFP expression cells, that corelated with the pattern of the path /spread of fluid produced by the three delivery systems. The number and intensity cell expressing eGFP was intense in mice injecting with hypodermic syring compare to Injex and Twin-Jector EZII. - The results seen with India ink support the eGFP expression results. In conclusion, needle free injection can be used to deliver DNA into BALB/c muscle, but less efficient compared to hypodermic syringe.
AB - The capability of DNA vaccine to transfect cells determines the success of DNA vaccine, but there are some natural obstacles that reduce the efficiency of DNA delivery into cells. One way to overcome this problem is through the use of a delivery systems such as needle free injectors (NFIT). In the present study, we evaluated 2 kinds of NFITs, Injex and Twin-Jector EZII, and as control we use hypodermic syringes. The path of injection and the capability of NFITs to deliver DNA into cells were observed by using India ink and plasmid coding enhanced green fluorescent protein (pCDNA eGFP) respectively. A mass of 50 μg pcDNA3.1-eGFP in 50 μL of PBS and India ink were injected into the mouse thigh using these three delivery systems. Two days post-injection, histological slides were prepared from the thigh muscle and the expression of eGFP was observed by confocal microscopy. The results show that all the delivery systems used in the present study were able to deliver India ink and pcDNA3.1-eGFP to the thigh muscle. There was a difference in the spread of Indian ink produced 3 kinds of delivery systems used in this study. Furthermore, there was also a difference in the distribution of eGFP expression cells, that corelated with the pattern of the path /spread of fluid produced by the three delivery systems. The number and intensity cell expressing eGFP was intense in mice injecting with hypodermic syring compare to Injex and Twin-Jector EZII. - The results seen with India ink support the eGFP expression results. In conclusion, needle free injection can be used to deliver DNA into BALB/c muscle, but less efficient compared to hypodermic syringe.
UR - http://www.scopus.com/inward/record.url?scp=85072599864&partnerID=8YFLogxK
U2 - 10.1063/1.5125537
DO - 10.1063/1.5125537
M3 - Conference contribution
AN - SCOPUS:85072599864
T3 - AIP Conference Proceedings
BT - Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
A2 - Mahat, Naji Arafat
A2 - Wahab, Roswanira Ab.
A2 - Huyop, Fahrul Zaman
A2 - Keyon, Aemi Syazwani Abdul
A2 - Attan, Nursyafreena Binti
A2 - Chandren, Sheela
A2 - Gunam, Ida Bagus Wayan
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
Y2 - 11 April 2019 through 12 April 2019
ER -
