TY - JOUR
T1 - The usage of enzyme in ultrasound-assisted enzymatic extraction method and its effect on yield extract from Keji Beling (Strobilanthes crispus.) leaves
AU - Angelina,
AU - Yumna, Mauhibah
AU - Abdullah,
AU - Arbianti, Rita
AU - Utami, Tania Surya
AU - Hermansyah, Heri
N1 - Publisher Copyright:
© The Authors, published by EDP Sciences, 2018.
PY - 2018/11/26
Y1 - 2018/11/26
N2 - Green extraction process is a method which is used to obtain various plant extracts with minimum impact on the environment. Green extraction will reduce energy consumption, allow use of alternative solvents and renewable natural products, and ensure a safe and high quality extract. Ultrasound-assisted enzymatic extraction (UAEE) is one of green extraction method. UAEE is a mild, efficient and environmental friendly extraction method and it has been adopted for extracting various kinds of compounds. The use of enzyme will catalyze hydrolysis of the cytoderm and glycoproteins, therefore enhancing the release of bioactive substances by disrupting plant cells. The optimum extraction conditions with a maximum yield extract of 48.63% are as follows: the concentration of ethanol is 50% and the amount of added enzyme is 70 mg/g. Crude extract from keji beling leaves is tested using Gas Chromatography and Mass Spectrometry (GC-MS) to identify components that have anti-hypercholesterolemic activity, which are hexadecanoic acid, octadecanoic acid and demethyl squalene.
AB - Green extraction process is a method which is used to obtain various plant extracts with minimum impact on the environment. Green extraction will reduce energy consumption, allow use of alternative solvents and renewable natural products, and ensure a safe and high quality extract. Ultrasound-assisted enzymatic extraction (UAEE) is one of green extraction method. UAEE is a mild, efficient and environmental friendly extraction method and it has been adopted for extracting various kinds of compounds. The use of enzyme will catalyze hydrolysis of the cytoderm and glycoproteins, therefore enhancing the release of bioactive substances by disrupting plant cells. The optimum extraction conditions with a maximum yield extract of 48.63% are as follows: the concentration of ethanol is 50% and the amount of added enzyme is 70 mg/g. Crude extract from keji beling leaves is tested using Gas Chromatography and Mass Spectrometry (GC-MS) to identify components that have anti-hypercholesterolemic activity, which are hexadecanoic acid, octadecanoic acid and demethyl squalene.
UR - http://www.scopus.com/inward/record.url?scp=85058685507&partnerID=8YFLogxK
U2 - 10.1051/e3sconf/20186703002
DO - 10.1051/e3sconf/20186703002
M3 - Conference article
AN - SCOPUS:85058685507
SN - 2555-0403
VL - 67
JO - E3S Web of Conferences
JF - E3S Web of Conferences
M1 - 03002
T2 - 3rd International Tropical Renewable Energy Conference "Sustainable Development of Tropical Renewable Energy", i-TREC 2018
Y2 - 6 September 2018 through 8 September 2018
ER -
