The usage of enzyme in ultrasound-assisted enzymatic extraction method and its effect on yield extract from Keji Beling (Strobilanthes crispus.) leaves

Angelina, Mauhibah Yumna, Abdullah, Rita Arbianti, Tania Surya Utami, Heri Hermansyah

Research output: Contribution to journalConference articlepeer-review

Abstract

Green extraction process is a method which is used to obtain various plant extracts with minimum impact on the environment. Green extraction will reduce energy consumption, allow use of alternative solvents and renewable natural products, and ensure a safe and high quality extract. Ultrasound-assisted enzymatic extraction (UAEE) is one of green extraction method. UAEE is a mild, efficient and environmental friendly extraction method and it has been adopted for extracting various kinds of compounds. The use of enzyme will catalyze hydrolysis of the cytoderm and glycoproteins, therefore enhancing the release of bioactive substances by disrupting plant cells. The optimum extraction conditions with a maximum yield extract of 48.63% are as follows: the concentration of ethanol is 50% and the amount of added enzyme is 70 mg/g. Crude extract from keji beling leaves is tested using Gas Chromatography and Mass Spectrometry (GC-MS) to identify components that have anti-hypercholesterolemic activity, which are hexadecanoic acid, octadecanoic acid and demethyl squalene.

Original languageEnglish
Article number03002
JournalE3S Web of Conferences
Volume67
DOIs
Publication statusPublished - 26 Nov 2018
Event3rd International Tropical Renewable Energy Conference "Sustainable Development of Tropical Renewable Energy", i-TREC 2018 - Kuta, Bali, Indonesia
Duration: 6 Sep 20188 Sep 2018

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