@inproceedings{b55e109ad9cf4bce920cff629d0d717f,
title = "The study on in vitro formation of DNA adduct 8-hydroxy-2-deoxyduanosine (8-OHdG) from benzo[a]pyrene and Fenton-like reaction",
abstract = "Cigarette is one of the factors that increases the numbers of human deaths because it contains many harmful substances, such as benzo[a]pyren (B[a]P) and heavy metal Cr(VI). One of the consequences caused by both substances is an oxidative DNA damage and DNA adducts 8-hydroxy-2'-deoxyguanosine (8-OHdG) production as a biomarker of cancer risk. In this research, in vitro assay of benzo[a]pyrene with Fenton-like reagent was conducted in several pHs (7.4 and 8.4), temperatures (37°C and 60°C), and incubation times (5 and 7 hours). The 8-OHdG DNA adducts was analyzed using a reverse phase HPLC chromatography with UV/vis detector at the wavelength of 254 nm. The result shows that the 8-OHdG concentration due to exposure of B[a]P in the presence of Fenton-like reagent has exceeded the minimum concentration of detection that was set as LOD value of 5.188 ppb. Based on the data result, the increasing time and temperature incubation have a synergistic effect of the 8-OHdG-concentration increase. The highest concentration of 8-OHdG formation was conducted in the condition of pH 7.4, temperature 60°C, and incubation time 12 h in the presence of B[a]P, Cr(VI), H2O2 and vitamin C as much as 15.13 ppb.",
keywords = "8-OHdG, Benzo[α]pyrene, Cr(VI), DNA Adduct, Fenton-like Reaction",
author = "B. Budiawan and Putri, {D. O.} and S. Handayani and Dani, {I. C.} and R. Bakri",
note = "Funding Information: Authors would like to thank Universitas Indonesia for funding this project through PITTA Grant 2017 and Balai K3 Hiperkes for facilitating the HPLC instrument. Publisher Copyright: {\textcopyright} 2018 Author(s).; 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 ; Conference date: 26-07-2017 Through 27-07-2017",
year = "2018",
month = oct,
day = "22",
doi = "10.1063/1.5064107",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Ratna Yuniati and Terry Mart and Anggraningrum, {Ivandini T.} and Djoko Triyono and Sugeng, {Kiki A.}",
booktitle = "Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017",
}