The study on in vitro formation of DNA adduct 8-hydroxy-2-deoxyduanosine (8-OHdG) from benzo[a]pyrene and Fenton-like reaction

Budiawan, D. O. Putri, Sri Handayani, I. C. Dani, Ridla Bakri

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cigarette is one of the factors that increases the numbers of human deaths because it contains many harmful substances, such as benzo[a]pyren (B[a]P) and heavy metal Cr(VI). One of the consequences caused by both substances is an oxidative DNA damage and DNA adducts 8-hydroxy-2'-deoxyguanosine (8-OHdG) production as a biomarker of cancer risk. In this research, in vitro assay of benzo[a]pyrene with Fenton-like reagent was conducted in several pHs (7.4 and 8.4), temperatures (37°C and 60°C), and incubation times (5 and 7 hours). The 8-OHdG DNA adducts was analyzed using a reverse phase HPLC chromatography with UV/vis detector at the wavelength of 254 nm. The result shows that the 8-OHdG concentration due to exposure of B[a]P in the presence of Fenton-like reagent has exceeded the minimum concentration of detection that was set as LOD value of 5.188 ppb. Based on the data result, the increasing time and temperature incubation have a synergistic effect of the 8-OHdG-concentration increase. The highest concentration of 8-OHdG formation was conducted in the condition of pH 7.4, temperature 60°C, and incubation time 12 h in the presence of B[a]P, Cr(VI), H2O2 and vitamin C as much as 15.13 ppb.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • 8-OHdG
  • Benzo[α]pyrene
  • Cr(VI)
  • DNA Adduct
  • Fenton-like Reaction

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