The sensitivity of selective veillonella medium as confirmed by gram staining and veill-rpob polymerase chain reaction

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Abstract

The number of Veillonella bacteria in the oral cavity is suggested to be a biological indicator and early warning sign of oral acid production, and therefore may help to predict the development of imminent dental caries. A simple technique is recommended in the present study, which aimed to assess the sensitivity of the approach. The potential use of Rogosa agar as a Veillonella-selective growth medium was tested by incubating the microbes in an anaerobic atmosphere for several days. Afterwards, the colony and cellular morphology of 173 Rogosa agar plates were observed under light microscope after Gramstaining. The presence of microbes was confirmed using the polymerase chain reaction (PCR) technique, which detects genuses with Veill-rpoB primer. Gram-stain observation revealed Gram-negative cocci, which underwent DNA extraction and Veillonella genus identification viaPCR. As a Veillonella-selective medium, Rogosa agar has 80.3% selectivity, as determined by Gram-negative cocci (100% of which were members of the Veillonella genus) and confirmed by PCR. Rogosa agar's sensitivity to Veillonella was 80.3%.

Original languageEnglish
Pages (from-to)764-768
Number of pages5
JournalJournal of International Dental and Medical Research
Volume10
Issue numberSpecialissue
Publication statusPublished - 1 Jan 2017

Keywords

  • Veill-rpoB PCR
  • Veillonella spp
  • Veillonella-selective medium

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