THE ROLE OF PROPOLIS TETRAGONULA SP. IN OXIDATIVE STRESS AND ITS PROTECTIVE EFFECT AGAINST UV RADIATION ON CELLS

Safira Candra Asih, Diah Kartika Pratami, Masafuhmi Yohda, Yosuke Fukutami, Ahmad Faried, Muhamad Sahlan

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: This report presents a study the anti-oxidant properties of propolis wax from Tetragonula sp. bees by evaluating the protection ability of propolis towards cells from ultraviolet (UV) exposure. The next evaluation was using a literature study and LC-MS (Liquid Chromatography-Mass Spectrometry) to find compounds in propolis that are responsible for anti-oxidative stress activity. Methods: HEK 293T (Human Embryonic Kidney 293 T) and fibroblast cells line were used. Four tests were performed on the cells, namely: cell proliferation assay using water-soluble tetrazolium salt (WST-8); lactate dehydrogenase (LDH), and free radicals produced on cells test by measuring fluorescence intensity produced by dichlorofluorescein; cell viability through observation using fluorescence microscope on cells stained with Hoechst and propidium iodide (PI); and reactive oxygen assay (ROS) Assay. Before UV exposure, propolis wax was added to the cells in different concentrations. The authors also analyzed the component in propolis wax using LC-MS. Results: Based on this assessment, it was found that propolis wax successfully protects the cells against UV-induced free radicals’ formation by maintaining the cell proliferation rate, reducing the free radicals produced after UV exposure, and decreasing the number of cell death. Nevertheless, we found that a greater concentration of propolis wax tends to be toxic to the cells. While on the LC-MS results obtained about 83 compounds, in which 35 of them are flavonoid and polyphenols derived compounds with antioxidant properties. Conclusion: Based on these findings, propolis wax produced Tetragonula sp. can be used as a potential alternative treatment of anti-oxidative stress and anti-free radicals.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalInternational Journal of Applied Pharmaceutics
Volume14
Issue numberSpecial Issue 3
DOIs
Publication statusPublished - Jun 2022

Keywords

  • Anti-oxidant
  • Free radicals
  • Oxidative stress
  • Propolis

Fingerprint

Dive into the research topics of 'THE ROLE OF PROPOLIS TETRAGONULA SP. IN OXIDATIVE STRESS AND ITS PROTECTIVE EFFECT AGAINST UV RADIATION ON CELLS'. Together they form a unique fingerprint.

Cite this