Background: Many studies had been conducted regarding the association of human papillomavirus (HPV) with laryngeal cancer. HPV was assumed to be one of the etiology of squamous cell carcinoma (SCC) besides smoking and alcohol consumption. Neck lymph node metastasis which is found in advanced laryngeal cancer could decrease the 5-year survival rate up to 50%. Purpose: This study aimed to investigate the role of HPV infections in the oncogenesis of the advanced laryngeal SCC and to evaluate the role of HPV in neck metastasis. Methods: Cross-sectional, double blind study with planned data collection. Data were taken from Formalin Fixed Paraffin Embedded (FFPE) of laryngeal cancer specimen after laryngectomy. Samples were analyzed by nested Polymerase Chain Reaction (PCR) and continuous flow-through hybridization for genotyping. Expression of Epidermal Growth Factor Receptor (EGFR), and Vascular Endothelial Growth Factor (VEGF) as metastasis biomarker were evaluated by immunohistochemistry. Results: Overall HPV proportion in laryngeal cancer was 28.7%. A total of 9% laryngeal cancer patients were infected with high risk HPV type and HPV 16 was found in 5 out of 7 samples. Mantel-Haenszel multivariate analysis found that HPV infection did not play a role in neck metastasis even though there were positive evidences of metastasis biomarker. On the contrary, in the absent of HPV, high expression metastasis biomarkers increased the risk of neck nodes metastasis: in EGFR 3.38 and VEGF 5.14 fold. Conclusion: HPV was found to be an oncogenic factor of laryngeal SCC, and HPV 16 was the most frequently observed type of HPV. HPV had protective function towards lymph node metastasis.