The role of cigarettes smoke condensatein enhanced candida albicans virulence of salivary isolates based on time and temperature

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Abstract

Candida albicans is a commensal of the oral cavity and the main agent of oral candidiasis. Cigarette smoke is reported to bea predisposing factor for biofilm formation and morphological changes of C. albicans. This study aimed to analyze the role of cigarette smoke condensate (CSC) on biofilm formation and morphological changes of C. albicans saliva isolates. C. albicans isolated from a smoker's saliva was cultured on CHROM-agar and standardizedusing a 0.5 McFarland (1×108 CFU/ml). The biofilms were assayed using spectrophotometric density (620 nm wavelength). The data analyzed by one-way analysis of variance (ANOVA) alsoPearson'scorrelatinwasused. The biofilm mass and morphological changes of C. albicans cells were observed by light microscopy (magnificationx 1000). The results showed that non-Indonesian tobacco (non-kretek) CSC strongly induced the formation of biofilms compared to kretek CSC, particularly at 24, 48, and 72 hours (p <0.05) compared to 12hours (p <0.01). This was in accordance with the biofilm mass observed by light microscopy and consistent with the transition of morphological changes from blastospora to pseudohypha and hypha (p <0.05). Kretek and non-kretek CSC increased the biofilm formation and morphological transition changes of C. albicans saliva isolates.

Original languageEnglish
Pages (from-to)769-777
Number of pages9
JournalJournal of International Dental and Medical Research
Volume10
Issue numberSpecialissue
Publication statusPublished - 1 Jan 2017

Keywords

  • Biofilm
  • Candida albicans
  • Cigarette smoke condensate
  • Morphology change

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