The role of anti-dengue virus NS-1 and anti-protein disulfide isomerase antibodies on platelet aggregation in secondary dengue infection

Andhika Rachman, A. R. Harahap, R. M. Widhyasih

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

AIM: to observe the correlation between anti-NS-1 and anti PDI antibodies against platelets function disorder on secondary dengue infection.

METHODS: 50 patients with secondary DV infection according to WHO criteria were observed by a cross sectional study. Patient's blood was collected on day 3, 5 and 7 after fever onset. Platelets aggregation test was done to prove the possibility of platelets dysfunction. Anti-NS-1 and anti-PDI antibodies were determined by solid phase ELISA.

RESULTS: the inhibition of platelets aggregation was increased among day of observation. Means value of inhibition on day 3 is 46.6%, day 5 is 52.5% and day 7 is 56%. There is a significant difference (p<0.05) of inhibition of platelet aggregation value between days of observation. The antibodies against NS-1 DV and PDI were detected in all 50 sera with the positive rate of 90% develop NS-1 antibodies and 72% of PDI antibodies, on day 3 of symptoms. The highest OD of NS-1 antibodies is detected on the day 3 and decreased on day 7. The OD of PDI antibodies was increased on day 3 and still increasing on day 7. There is a significant correlation between anti NS-1 and PDI antibodies (r=0.386-0.490), while the differences of OD between observation days are not significant (p>0.05).

CONCLUSION: the kinetics profile of NS1 and PDI antibodies responses, which were detected by the third day of symptoms. Dengue patients' sera inhibited platelets aggregation. NS-1 antibodies and PDI antibodies might have a role on the platelets aggregation dysfunction; however, there is no correlation between them. It is possible that other mechanism involve in the inhibition of platelets aggregation.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalActa medica Indonesiana
Volume45
Issue number1
Publication statusPublished - 1 Jan 2013

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