The qualitative screening of cellulolytic, chitinolytic, IAA-producing, and phosphate solubilizing bacteria from black soldier fly larvae (Hermetia illucens L.)

H. Tamrela, A. Sugiyanto, I. Santoso, Q. G. Fadhilah

Research output: Contribution to journalConference articlepeer-review

Abstract

Black soldier fly (Hermetia illucens L.) larvae are widely used in organic waste management. Its ability to digest organic compound was supported by the diversity of bacteria which produce various enzymes inside the larvae bodies. The exploration of potential bacteria in BSF larvae may have advantages for the agricultural field. This study aimed to screen cellulolytic, chitinolytic, phosphate solubilizing, and IAA-producing bacteria from BSF larvae. A total of 22 isolates were qualitatively screened using various media. Among the 22 isolates, five isolates showed positive results of cellulolytic activity; six isolates were able to digest chitin; nine isolates have positive result of IAA-producing test; and five isolates could solubilize phosphate in Pikovskaya's agar. There were two isolates, B11 and B15, that gave positive result in three tests, those are on cellulolytic, phosphate solubilizing, and IAA producing test. Molecular identification of B11 showed that the isolate was similar to Bacillus altitudinis (100%) and B15 was closed to Klebsiella oxytoca (99.73%).

Original languageEnglish
Article number012065
JournalIOP Conference Series: Earth and Environmental Science
Volume948
Issue number1
DOIs
Publication statusPublished - 16 Dec 2021
Event4th International Conference on Biosciences, ICoBio 2021 - Bogor, Indonesia
Duration: 11 Aug 202112 Aug 2021

Keywords

  • agriculture
  • Bacillus altitudinis
  • bacterial activities
  • Hermetiaillucens L. larvae
  • Klebsiella oxytoca

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