The Prospect of chitosan on the osteogenesis of periodontal ligament stem cells

Biomaterial innovation is needed for bone substitute which can help the osteogenesis process on periodontal tissue regeneration. This study is an experimental in-vitro study of different concentration of chitosan and aimed to analyze the osteogenesis of human periodontal ligament stem cells (PDLsc) cultured in chitosan. Forty eight PDLsc were collected from healthy and periodontitis third lower molar. There were 24 healthy teeth-PDLsc and 24 periodontitis teeth-PDLsc. Each groups were divided into three experimental groups and were layered by 200 μl concentration of chitosan (0.15%, 0.2%; 0.25%) and diluted in 0,5 % citric acid. Cell culture without chitosan was used as a control group. The levels of alkaline phosphatase (ALP), osteocalcin and calcium deposit in the cell culture were measured on day 3, day 7, day 14 and day 21. Chitosan at concentration of 0.25 % shows a potential impact of PDLsc in both periodontitis and healthy teeth samples, especially in the level of ALP and calcium deposit. Chitosan has potential effect in increasing of osteogenesis process in periodontal ligament cells for regeneration of periodontal tissues.