The prognostic significance of apoptosis-associated proteins Bcl-2, Bax and Bcl-X in clinical nephroblastoma

M. A. Ghanem, T. H.Van der Kwast, J. C.Den Hollander, Mondastri Korib Sudaryo, M. M.Van den Heuvel, M. A. Noordzij, R. J.M. Nijman, E. H. Soliman, G. J.van Steenbrugge

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Apoptotic cell death represents an important mechanism for the precise regulation of cell numbers in normal tissues. Various apoptosis-associated regulatory proteins, such as Bcl-2, Bax and Bcl-X, may contribute to the rate of apoptosis in neoplasia. The present study was performed to evaluate the prognostic value of these molecules in a group of 61 Wilms' tumours of chemotherapeutically pre-treated patients using an immunohistochemical approach. Generally, Bcl-2, Bax and for Bcl-XS/L were expressed in the blastemal and epithelial components of Wilms' tumour. Immunoreactive blastema cells were found in 53%, 41% and 38% of tumours for Bcl-2, Bax and for Bcl-XS/L, respectively. An increased expression of Bcl-2 was observed in the blastemal component of increasing pathological stages. In contrast, a gradual decline of Bax expression was observed in the blastemal component of tumours with increasing pathological stages. Also blastemal Bcl-XS/L expression decreased with stage. Univariate analysis showed that blastemal Bcl-2 expression and the Bcl-2/Bax ratio were indicative for clinical progression, whereas epithelial staining was of no prognostic value. Multivariate analysis showed that blastemal Bcl-2 expression is an independent prognostic marker for clinical progression besides stage. These findings demonstrate that alterations of the Bcl-2/Bax balance may influence the clinical outcome of Wilms' tumour patients by deregulation of programmed cell death.

Original languageEnglish
Pages (from-to)1557-1563
Number of pages7
JournalBritish Journal of Cancer
Volume85
Issue number10
DOIs
Publication statusPublished - 16 Nov 2001

Keywords

  • Bax
  • Bcl-2
  • Bcl-X
  • Prognosis
  • Wilms' tumour

Fingerprint Dive into the research topics of 'The prognostic significance of apoptosis-associated proteins Bcl-2, Bax and Bcl-X in clinical nephroblastoma'. Together they form a unique fingerprint.

Cite this