@inproceedings{b45e589647db43089ba24f4e03164a22,
title = "The profile of ovarian tissue of female Sprague-Dawley rats (Rattus norvegicus L.) after vitrification using ethylene glycol and egg yolk as cryoprotectant",
abstract = "Vitrification has been widely used to preserve ovarian tissue. However, an efficient method with minimal damaging effect towards the tissue has not been established, yet. Accordingly, the combination method among intra- and extracellular cryoprotectant was conducted in this study. The aim of the research is to evaluate combination of ethylene glycol (EG) as intracellular cryoprotectant with egg yolk as extracellular cryoprotectant in reducing coldshock effect on ovarian tissue vitrification after 48 hours. Twenty one samples of twelve week-old female Sprague-Dawley rats are used in this study. Ovarian samples (n = 21) were washed in saline to clear excessive blood and were weighed. Samples for control groups (n = 3) were immediately fixated. Control treatment groups (n = 9) plunged into cryotube filled with 3.75 %, 7.5 % and 15 % of EG solution, respectively, while treatment groups (n = 9) were 3.75 %, 7.5 % and 15 % of egg yolk+EG solution, respectively. All samples were then frozen in liquid nitrogen (- 196 °C). After 48 hours, the samples were thawed and weighed. The result shown that based on Anova test there were no significant different (p<0.05) in weight of ovarian tissue before and after vitrification. In conclusion, vitrification using a combination of ethylene glycol (EG) and egg yolk may have significant impact in preventing coldshock in the ovary.",
keywords = "Egg yolk, ethylene glycol, female rats, ovarian tissue, vitrification",
author = "A. Awanis and N. Anita and A. Abinawanto and Jusuf, {A. A.} and I. Muhiardi and Rosvita, {M. A.} and A'Yun, {Y. Q.}",
note = "Funding Information: The authors would like to send our gratitude to Universitas Indonesia and Directorate of Research and Public Service (DRPM) UI for providing financial assistance by Hibah Publikasi Internasional Terindeks untuk Tugas Akhir (PITTA) UI 2018. We would like to thank to researchers and staffs of Health Research and Development Agency of Ministry of Health and the Laboratory of Animal Physiology of Biology Department, Universitas Indonesia for their courtesy and assistance. Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 ; Conference date: 30-10-2018 Through 31-10-2018",
year = "2019",
month = nov,
day = "4",
doi = "10.1063/1.5132524",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Terry Mart and Djoko Triyono and Anggraningrum, {Ivandini T.}",
booktitle = "Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018",
address = "United States",
}