The profile of ovarian tissue after vitrification using ethylene glycol (EG) and longan honey in female Sprague-Dawley rats (Rattus norvegicus L.)

I. Muhiardi, N. Anita, A. Abinawanto, A. A. Jusuf, A. Awanis, M. A. Rosvita, Y. Q. A'Yun

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Ethylene Glycol (EG) has been known as cryoprotectant solution for vitrification of biological organs that are widely used. However, using other cryoprotectants that are comparable to EG that are safer to use is considered. The aim of this study is to evaluate the effectiveness of rat (Rattus norvegicus L.) Sprague-Dawley strain ovarian tissue after 48 hours post-vitrification by using a mixture of EG and longan honey as cryoprotectant in various concentrations (3.75 %, 7.5 %, 15 %). The ovaries (n = 21) were taken to determine the mass parameters and are divided into 3 groups: non-vitrified control (n = 3) which are only fixated using alcohol, treatment control (n = 9) which uses 3.75 %, 7.5 %, 15 % EG solution, and treatment group (n = 9) which uses 3.75 %, 7.5 %, 15 % combination of longan honey and EG solution. The ANAVA and LSD test analysis showed that between pre-vitrification (P = 0.807) and post-vitrification (P = 0.366) ovaries that used various concentrations of longan honey did not give any significant results. In conclusion, EG and honey did not affect the physical profile of ovarian tissue after vitrification.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
Country/TerritoryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • ethylene glycol
  • Longan honey
  • ovary
  • vitrification

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