Ethylene Glycol (EG) has been known as cryoprotectant solution for vitrification of biological organs that are widely used. However, using other cryoprotectants that are comparable to EG that are safer to use is considered. The aim of this study is to evaluate the effectiveness of rat (Rattus norvegicus L.) Sprague-Dawley strain ovarian tissue after 48 hours post-vitrification by using a mixture of EG and longan honey as cryoprotectant in various concentrations (3.75 %, 7.5 %, 15 %). The ovaries (n = 21) were taken to determine the mass parameters and are divided into 3 groups: non-vitrified control (n = 3) which are only fixated using alcohol, treatment control (n = 9) which uses 3.75 %, 7.5 %, 15 % EG solution, and treatment group (n = 9) which uses 3.75 %, 7.5 %, 15 % combination of longan honey and EG solution. The ANAVA and LSD test analysis showed that between pre-vitrification (P = 0.807) and post-vitrification (P = 0.366) ovaries that used various concentrations of longan honey did not give any significant results. In conclusion, EG and honey did not affect the physical profile of ovarian tissue after vitrification.