The production of bioinsecticide based from Pong-Pong fruit seed extract by ultrasonic waved extraction using nades solvent

Cutworms population is increasing rapidly and may damage crops in Indonesia. Therefore, control of those pests is needed using bioinsecticides obtained from Pong-Pong fruit seed extract. These seeds have an active ingredient from alkaloid and steroid groups that can eliminate the worms. The seeds are prepared by mashing and mixing them with a Natural Deep Eutectic Solvent (NADES) and undergo ultrasonic-assisted extraction (UAE) with different concentration and sonication times to obtain the most optimal one by checking the most lethal extract given to the worms. Tests using Liebermann-Burchard, FTIR and LCMS have identified the components of the extract.