The prevalence of oral high-risk HPV infection in Indonesian oral squamous cell carcinoma patients

Denni J. Purwanto; Nurtami Soedarsono; Jauhari O. Reuwpassa; Asri C. Adisasmita; Muchlis Ramli; Ratna Djuwita

doi:10.1111/odi.13221

The prevalence of oral high-risk HPV infection in Indonesian oral squamous cell carcinoma patients

Denni J. Purwanto, Nurtami Soedarsono, Jauhari O. Reuwpassa, Asri C. Adisasmita, Muchlis Ramli, Ratna Djuwita

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Objectives: The objective of this study was to report the integrated observations of high-risk HPV-related oral squamous carcinoma (OSCC) at our national referral center for cancer, the Dharmais National Cancer Hospital (DNCH), Jakarta, from 2003 to 2013. Materials and Methods: Seventy-eight formalin-fixed paraffin-embedded specimens obtained from OSCC cases were collected from 2003 to 2013 DNCH archives and were included in this high-risk HPV (HR-HPV) study. Seventy-nine DNA samples from the normal oral mucosa of healthy individuals were obtained from the Oral Biology Laboratory DNA archives from 2001 to 2005. Glyceraldehyde 3-phosphate dehydrogenase was used as a control to ensure the DNA integrity for the subsequent HPV DNA PCR detection. High-risk HPV16/18 DNA amplification was conducted by nested PCR using two pairs of primers that were designed specifically to identify the region of gene L1 HPV16 and the HPV16/18 region. Results and Conclusions: A high prevalence of HPV16/18 was detected in OSCC cases (17.9%). HPV18 occurred more often than HPV16 (86%) among OSCC patients who were HPV positive. This result supports high HPV18 prevalence among Indonesian cervical cancer patients studied in 1995 and 2006. The prevalence of high-risk HPV remains low in the normal Indonesian population (3.8%), but HPV16 is consistently more frequently detected in non-cancer populations.

Original language	English
Pages (from-to)	72-80
Number of pages	9
Journal	Oral Diseases
Volume	26
Issue number	1
DOIs	https://doi.org/10.1111/odi.13221
Publication status	Published - 1 Jan 2020

Keywords

HPV16/18
Oral Cancer
prevalence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/odi.13221

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title = "The prevalence of oral high-risk HPV infection in Indonesian oral squamous cell carcinoma patients",

abstract = "Objectives: The objective of this study was to report the integrated observations of high-risk HPV-related oral squamous carcinoma (OSCC) at our national referral center for cancer, the Dharmais National Cancer Hospital (DNCH), Jakarta, from 2003 to 2013. Materials and Methods: Seventy-eight formalin-fixed paraffin-embedded specimens obtained from OSCC cases were collected from 2003 to 2013 DNCH archives and were included in this high-risk HPV (HR-HPV) study. Seventy-nine DNA samples from the normal oral mucosa of healthy individuals were obtained from the Oral Biology Laboratory DNA archives from 2001 to 2005. Glyceraldehyde 3-phosphate dehydrogenase was used as a control to ensure the DNA integrity for the subsequent HPV DNA PCR detection. High-risk HPV16/18 DNA amplification was conducted by nested PCR using two pairs of primers that were designed specifically to identify the region of gene L1 HPV16 and the HPV16/18 region. Results and Conclusions: A high prevalence of HPV16/18 was detected in OSCC cases (17.9%). HPV18 occurred more often than HPV16 (86%) among OSCC patients who were HPV positive. This result supports high HPV18 prevalence among Indonesian cervical cancer patients studied in 1995 and 2006. The prevalence of high-risk HPV remains low in the normal Indonesian population (3.8%), but HPV16 is consistently more frequently detected in non-cancer populations.",

keywords = "HPV16/18, Oral Cancer, prevalence",

author = "Purwanto, {Denni J.} and Nurtami Soedarsono and Reuwpassa, {Jauhari O.} and Adisasmita, {Asri C.} and Muchlis Ramli and Ratna Djuwita",

note = "Funding Information: Sociodemographic and clinicopathologic information was only collected retrospectively from the medical records of OSCC patients. Patient's characteristics included age and sex, whereas clinicopathologic data included site of OSCC, tumor characteristics (tumor, node, metastasis), stage, differentiation of the tumor cell, underwent chemotherapy and/or radiotherapy, and the presence of comorbid conditions. No sociodemographic information was available for the healthy individuals. This study has been reviewed and approved by the Committee of the Medical Research Ethics of the DNCH No: KEPK/041/X/2015 and The Committee of Public Health Faculty, University of Indonesia No: 250/UN2.F10/PPM.00.02/2015. Funding Information: We are grateful to the Department of Research Development and the Department of Pathology Anatomy of DNCH for their grant support and laboratory assistance. Funding Information: We are grateful to the Department of Research Development and the Department of Pathology Anatomy of DNCH for their grant support and laboratory assistance. Publisher Copyright: {\textcopyright} 2019 The Authors. Oral Diseases published by John Wiley & Sons Ltd",

year = "2020",

month = jan,

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doi = "10.1111/odi.13221",

language = "English",

volume = "26",

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journal = "Oral Diseases",

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AU - Purwanto, Denni J.

AU - Soedarsono, Nurtami

AU - Reuwpassa, Jauhari O.

AU - Adisasmita, Asri C.

AU - Ramli, Muchlis

AU - Djuwita, Ratna

N1 - Funding Information: Sociodemographic and clinicopathologic information was only collected retrospectively from the medical records of OSCC patients. Patient's characteristics included age and sex, whereas clinicopathologic data included site of OSCC, tumor characteristics (tumor, node, metastasis), stage, differentiation of the tumor cell, underwent chemotherapy and/or radiotherapy, and the presence of comorbid conditions. No sociodemographic information was available for the healthy individuals. This study has been reviewed and approved by the Committee of the Medical Research Ethics of the DNCH No: KEPK/041/X/2015 and The Committee of Public Health Faculty, University of Indonesia No: 250/UN2.F10/PPM.00.02/2015. Funding Information: We are grateful to the Department of Research Development and the Department of Pathology Anatomy of DNCH for their grant support and laboratory assistance. Funding Information: We are grateful to the Department of Research Development and the Department of Pathology Anatomy of DNCH for their grant support and laboratory assistance. Publisher Copyright: © 2019 The Authors. Oral Diseases published by John Wiley & Sons Ltd

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Objectives: The objective of this study was to report the integrated observations of high-risk HPV-related oral squamous carcinoma (OSCC) at our national referral center for cancer, the Dharmais National Cancer Hospital (DNCH), Jakarta, from 2003 to 2013. Materials and Methods: Seventy-eight formalin-fixed paraffin-embedded specimens obtained from OSCC cases were collected from 2003 to 2013 DNCH archives and were included in this high-risk HPV (HR-HPV) study. Seventy-nine DNA samples from the normal oral mucosa of healthy individuals were obtained from the Oral Biology Laboratory DNA archives from 2001 to 2005. Glyceraldehyde 3-phosphate dehydrogenase was used as a control to ensure the DNA integrity for the subsequent HPV DNA PCR detection. High-risk HPV16/18 DNA amplification was conducted by nested PCR using two pairs of primers that were designed specifically to identify the region of gene L1 HPV16 and the HPV16/18 region. Results and Conclusions: A high prevalence of HPV16/18 was detected in OSCC cases (17.9%). HPV18 occurred more often than HPV16 (86%) among OSCC patients who were HPV positive. This result supports high HPV18 prevalence among Indonesian cervical cancer patients studied in 1995 and 2006. The prevalence of high-risk HPV remains low in the normal Indonesian population (3.8%), but HPV16 is consistently more frequently detected in non-cancer populations.

AB - Objectives: The objective of this study was to report the integrated observations of high-risk HPV-related oral squamous carcinoma (OSCC) at our national referral center for cancer, the Dharmais National Cancer Hospital (DNCH), Jakarta, from 2003 to 2013. Materials and Methods: Seventy-eight formalin-fixed paraffin-embedded specimens obtained from OSCC cases were collected from 2003 to 2013 DNCH archives and were included in this high-risk HPV (HR-HPV) study. Seventy-nine DNA samples from the normal oral mucosa of healthy individuals were obtained from the Oral Biology Laboratory DNA archives from 2001 to 2005. Glyceraldehyde 3-phosphate dehydrogenase was used as a control to ensure the DNA integrity for the subsequent HPV DNA PCR detection. High-risk HPV16/18 DNA amplification was conducted by nested PCR using two pairs of primers that were designed specifically to identify the region of gene L1 HPV16 and the HPV16/18 region. Results and Conclusions: A high prevalence of HPV16/18 was detected in OSCC cases (17.9%). HPV18 occurred more often than HPV16 (86%) among OSCC patients who were HPV positive. This result supports high HPV18 prevalence among Indonesian cervical cancer patients studied in 1995 and 2006. The prevalence of high-risk HPV remains low in the normal Indonesian population (3.8%), but HPV16 is consistently more frequently detected in non-cancer populations.

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KW - prevalence

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U2 - 10.1111/odi.13221

DO - 10.1111/odi.13221

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AN - SCOPUS:85075729138

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SP - 72

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JO - Oral Diseases

JF - Oral Diseases

IS - 1

ER -

The prevalence of oral high-risk HPV infection in Indonesian oral squamous cell carcinoma patients

Abstract

Keywords

UN SDGs

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