TY - JOUR
T1 - The potential of basal medium astemporary prolong culture of umbilical cord derived mesenchymalstem cells
AU - Pawitan, Jeanne Adiwinata
AU - Kispa, Tera Dria
AU - Liem, Isabella Kurnia
AU - Mujadid, Fajar
AU - Novialdi, Novialdi
N1 - Publisher Copyright:
© 2018 Oriental Scientific Publishing Company.
PY - 2018/12
Y1 - 2018/12
N2 - To compare the viability and characteristics of umbilical cord mesenchymal stromal cells (UC-MSCs) when they were kept in culture in three types of áMEM after confluence for various time points. Viability and characteristic assessments were done after keeping UC-MSC confluent cultures for 0, 24, 48, 72, and 96hours in áMEM with glutamax (Himedia), and without glutamax (Himedia or Gibco).There were thirty cultures, which each ten of them were kept in the three types of basal media, and for each time point and type of basal medium, two flasks were harvested. All viability and characteristic assessments were done after harvest. Viability assessments were done in duplicate, while characteristics assessment by flowcytometry for CD73, CD90 and CD105 were done only once. Further, differences between the various time points in terms of viability and CD73, CD90 and CD105 percentage after various time points were compared and tested by appropriate statistical analysis. Viability was >70% upto 72 hours and 48 hours when the cells were kept in Himediaglutamax containing áMEM and áMEM without glutamax respectively. Flow cytometry showed that CD73, CD90 and CD negative percentage did not differ to initial percentage, but after 24 hours CD105 was slightly decreased to > 60% in Himediaglutamax containing áMEM, while in the two áMEM media without glutamaxthe percentages were below 40%. For our UC-MSC culture, glutamax containing áMEM is better compared to áMEM without glutamax as temporary storage solution, and storage should be restricted to 24 hours.
AB - To compare the viability and characteristics of umbilical cord mesenchymal stromal cells (UC-MSCs) when they were kept in culture in three types of áMEM after confluence for various time points. Viability and characteristic assessments were done after keeping UC-MSC confluent cultures for 0, 24, 48, 72, and 96hours in áMEM with glutamax (Himedia), and without glutamax (Himedia or Gibco).There were thirty cultures, which each ten of them were kept in the three types of basal media, and for each time point and type of basal medium, two flasks were harvested. All viability and characteristic assessments were done after harvest. Viability assessments were done in duplicate, while characteristics assessment by flowcytometry for CD73, CD90 and CD105 were done only once. Further, differences between the various time points in terms of viability and CD73, CD90 and CD105 percentage after various time points were compared and tested by appropriate statistical analysis. Viability was >70% upto 72 hours and 48 hours when the cells were kept in Himediaglutamax containing áMEM and áMEM without glutamax respectively. Flow cytometry showed that CD73, CD90 and CD negative percentage did not differ to initial percentage, but after 24 hours CD105 was slightly decreased to > 60% in Himediaglutamax containing áMEM, while in the two áMEM media without glutamaxthe percentages were below 40%. For our UC-MSC culture, glutamax containing áMEM is better compared to áMEM without glutamax as temporary storage solution, and storage should be restricted to 24 hours.
KW - Mesenchymal stem cell
KW - Population doubling time
KW - Storage solution
KW - Umbilical cord
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85062942514&partnerID=8YFLogxK
U2 - 10.13005/bpj/1557
DO - 10.13005/bpj/1557
M3 - Article
AN - SCOPUS:85062942514
SN - 0974-6242
VL - 11
SP - 1851
EP - 1855
JO - Biomedical and Pharmacology Journal
JF - Biomedical and Pharmacology Journal
IS - 4
ER -