The potential of basal medium astemporary prolong culture of umbilical cord derived mesenchymalstem cells

Jeanne Adiwinata, Tera Dria Kispa, Isabella Kurnia Liem, Fajar Mujadid, Novialdi Novialdi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

To compare the viability and characteristics of umbilical cord mesenchymal stromal cells (UC-MSCs) when they were kept in culture in three types of áMEM after confluence for various time points. Viability and characteristic assessments were done after keeping UC-MSC confluent cultures for 0, 24, 48, 72, and 96hours in áMEM with glutamax (Himedia), and without glutamax (Himedia or Gibco).There were thirty cultures, which each ten of them were kept in the three types of basal media, and for each time point and type of basal medium, two flasks were harvested. All viability and characteristic assessments were done after harvest. Viability assessments were done in duplicate, while characteristics assessment by flowcytometry for CD73, CD90 and CD105 were done only once. Further, differences between the various time points in terms of viability and CD73, CD90 and CD105 percentage after various time points were compared and tested by appropriate statistical analysis. Viability was >70% upto 72 hours and 48 hours when the cells were kept in Himediaglutamax containing áMEM and áMEM without glutamax respectively. Flow cytometry showed that CD73, CD90 and CD negative percentage did not differ to initial percentage, but after 24 hours CD105 was slightly decreased to > 60% in Himediaglutamax containing áMEM, while in the two áMEM media without glutamaxthe percentages were below 40%. For our UC-MSC culture, glutamax containing áMEM is better compared to áMEM without glutamax as temporary storage solution, and storage should be restricted to 24 hours.

Original languageEnglish
Pages (from-to)1851-1855
Number of pages5
JournalBiomedical and Pharmacology Journal
Volume11
Issue number4
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Mesenchymal stem cell
  • Population doubling time
  • Storage solution
  • Umbilical cord
  • Viability

Fingerprint Dive into the research topics of 'The potential of basal medium astemporary prolong culture of umbilical cord derived mesenchymalstem cells'. Together they form a unique fingerprint.

Cite this